Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) Suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P, Flow Cyt, WB and reacts with samples. Cited in 14 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ChIC/CUT&RUN-seq | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/70 | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Application ChIP-seq | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) Suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P, Flow Cyt, WB and reacts with samples. Cited in 14 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR25A
Affinity purification Protein A
Endotoxin level: <1EU/mg (based on Lumulus Amebocyte lysate assay).
Blue Ice
+4°C
Do Not Freeze
ab199376 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling KCTD5 with Anti-KCTD5 antibody [EPR16312] - C-terminal ab194825 at 1/70 dilution (red) compared with ab199376 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab199376 detects no signal in FC.
Note: ab199376 detects no signal in Flow cytometry.
Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (PE). Please refer to PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 for protocol details.
Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal [YOL1/34] to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeled with ab199376 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Confocal image showing no staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab199376 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093 for protocol details.
Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091 for protocol details.
Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Immunohistochemistry analysis of rat colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on rat colon tissue was observed.
Immunohistochemistry analysis of mouse colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on mouse colon tissue was observed.
Immunohistochemistry analysis of human colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on human colon tissue was observed.
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