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Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) Suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P, Flow Cyt, WB and reacts with samples. Cited in 14 publications.

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Images

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (AB199376), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Application

ChIC/CUT&RUN-seq

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IP

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

-

Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/70

Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Application

ChIP-seq

Reactivity

Expected

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) Suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P, Flow Cyt, WB and reacts with samples. Cited in 14 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Carrier free

Yes

Clone number

EPR25A

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Endotoxin level: <1EU/mg (based on Lumulus Amebocyte lysate assay).

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab199376 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling KCTD5 with Anti-KCTD5 antibody [EPR16312] - C-terminal ab194825 at 1/70 dilution (red) compared with ab199376 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab199376 detects no signal in FC.

    Note: ab199376 detects no signal in Flow cytometry.

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (PE). Please refer to PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 for protocol details.

    Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal [YOL1/34] to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeled with ab199376 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Confocal image showing no staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab199376 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093 for protocol details.

    Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Clone EPR25A (ab199376) has been successfully conjugated by Abcam. This image was generated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091 for protocol details.

    Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Immunohistochemistry analysis of rat colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on rat colon tissue was observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Immunohistochemistry analysis of mouse colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on mouse colon tissue was observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)

    Immunohistochemistry analysis of human colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on human colon tissue was observed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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