Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with samples. Cited in 4 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120425.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)")
![Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--flow-cytometry-intracellular-img6326.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)")
![Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunocytochemistry-immunofluorescence-img68547.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101446.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128471.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (AB208334)")
Publications
Filter by
- Signal transduction and targeted therapy 7:292022Integrated histopathological, lipidomic, and metabolomic profiles reveal mink is a useful animal model to mimic the pathogenicity of severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiqi Song,Linlin Bao,Wei Deng,Jiangning Liu,Erjun Ren,Qi Lv,Mingya Liu,Feifei Qi,Ting Chen,Ran Deng,Fengdi Li,Yunpeng Liu,Qiang Wei,Hong Gao,Pin Yu,Yunlin Han,Wenjie Zhao,Junjun Zheng,Xujian Liang,Fuhe Yang,Chuan QinPubMed 35091528
- Frontiers in microbiology 11:6188912021SARS-CoV-2 Causes a Systemically Multiple Organs Damages and Dissemination in Hamsters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiqi Song,Linlin Bao,Pin Yu,Feifei Qi,Shuran Gong,Jie Wang,Binbin Zhao,Mingya Liu,Yunlin Han,Wei Deng,Jiangning Liu,Qiang Wei,Jing Xue,Wenjie Zhao,Chuan QinPubMed 33510731
- Antioxidants (Basel, Switzerland) 9:2020Triple Combination of Ascorbate, Menadione and the Inhibition of Peroxiredoxin-1 Produces Synergistic Cytotoxic Effects in Triple-Negative Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalgorzata Bajor et. al.PubMed 32316111
- Cell death & disease 11:1242020Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristiano Guttà et. al.PubMed 32054850
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt (Intra) | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min. |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Alternative names
Ig gamma chain C region
Recommended products
Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Carrier free
Yes
- Clone number
SP137
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab208334 is the carrier-free version of Rabbit IgG, monoclonal [SP137] - isotype control ab125938 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab208334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120425.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Rabbit with Rabbit IgG, monoclonal [SP137] - isotype control ab125938 at 1/100 dilution (2.02 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--flow-cytometry-intracellular-img6326.jpg "Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling EPF with Anti-EPF antibody [EPR4475] ab108611 at 1:100 dilution (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [SP137] - isotype control ab125938) at 1:20 dilution (10.1 μg/ml)/ Black. Unlabeled control - Unlabelled cells / blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunocytochemistry-immunofluorescence-img68547.jpg "Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells showing staining with purified Rabbit IgG, monoclonal [SP137] - isotype control ab125938 at 1:20 (10 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101446.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling Rabbit with Rabbit IgG, monoclonal [SP137] - isotype control ab125938 at 1/100 dilution (2.02 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128471.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Rabbit with Rabbit IgG, monoclonal [SP137] - isotype control ab125938 at 1/100 dilution (2.02 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunocytochemistry-immunofluorescence-img77501.jpg "Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat ( human T cell leukemia T lymphocyte) cells showing staining with purified Rabbit IgG, monoclonal [SP137] - isotype control ab125938 at 1:20 (10 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [SP137] - isotype control ab125938).
![Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--western-blot-img430427.jpg "Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730).
Negative Control.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (Rabbit IgG, monoclonal [SP137] - isotype control ab125938) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Negative Control.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
![Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--immunocytochemistry-immunofluorescence-img77501.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
![Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334), expandable thumbnail](https://content.abcam.com/products/images/rabbit-igg-monoclonal-sp137-isotype-control-bsa-and-azide-free-ab208334--western-blot-img430427.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Rabbit IgG, monoclonal [SP137] - isotype control (BSA and Azide free) (ab208334)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com