Rabbit IgG, multiclonal [RM1060] - Isotype control - BSA and Azide free Suitable for ChIP-seq, Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP and reacts with samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Multiclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ChIP-seq | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt (Intra) | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Rabbit IgG, multiclonal [RM1060] - Isotype control - BSA and Azide free Suitable for ChIP-seq, Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP and reacts with samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Multiclonal
Yes
RM1060
Affinity purification Protein A
Blue Ice
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 4 µg of Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 4 µg of Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 4 µg of Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 [RM1060]. Assay Quality Control was conducted using 8 µg CTCF control Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408 on the same cell line. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing negative staining in C6 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing negative staining in NIH/3T3 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing negative staining in Hela cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on rat colon.The section was incubated with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on mouse colon.The section was incubated with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). No staining on human colon.The section was incubated with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in C6 (rat glial tumor glial cell) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-ADK antibody [EPR27497-67] ab307357 in C6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-ADK antibody [EPR27497-67] ab307357 using C6 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-ADK antibody [EPR27497-67] ab307357 in Lane 3.
All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) at 1/30 dilution
All lanes: C6 (rat glial tumor glial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in C6 (rat glial tumor glial cell) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-ADK antibody [EPR27497-67] ab307357 in C6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-ADK antibody [EPR27497-67] ab307357 using C6 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-ADK antibody [EPR27497-67] ab307357 in Lane 3.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using NIH/3T3 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.
All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using NIH/3T3 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg U-937 (human histiocytic lymphoma monocyte) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in U937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using U937 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.
All lanes: Immunoprecipitation - Rabbit IgG, multiclonal [RM1060] - Isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) at 1/30 dilution
All lanes: U-937 (human histiocytic lymphoma monocyte) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Rabbit IgG, multiclonal [RM1060] - Isotype control was immunoprecipitated from 0.35 mg U-937 (human histiocytic lymphoma monocyte) whole cell lysate with Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 2: Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 IP in U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 3: Rabbit IgG isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in U937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using U937 cells. Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801 was used as the isotype control instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in Lane 3.
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
This data was developed using Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 seconds
All lanes: Western blot - Anti-Rabbit IgG antibody [RM1060] -isotype control (Anti-Rabbit IgG antibody [RM1060] - Isotype Control ab313801) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 59s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 seconds
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