Rabbit polyclonal IgG isotype control antibody that is used to detect IgG in IHC-P, ICC/IF, Flow cytometryometry, ChIP, ELISA.
- An isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions
- Cited in over 270 publications
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- Gut 73:325-3372024is a risk haplotype for primary sclerosing cholangitis mediating activation of NKp44+ NK cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBritta F Zecher,David Ellinghaus,Sebastian Schloer,Annika Niehrs,Benedetta Padoan,Martin E Baumdick,Yuko Yuki,Maureen P Martin,Dawid Glow,Jennifer Schröder-Schwarz,Jennifer Niersch,Sébastien Brias,Luisa M Müller,Robin Habermann,Paul Kretschmer,Tristan Früh,Janis Dänekas,Malte H Wehmeyer,Tobias Poch,Marcial Sebode,Eva Ellinghaus,Frauke Degenhardt,Christian Körner,Angelique Hoelzemer,Boris Fehse,Karl J Oldhafer,Udo Schumacher,Guido Sauter,Mary Carrington,Andre Franke,Madeleine J Bunders,Christoph Schramm,Marcus AltfeldPubMed 37788895
- JCI insight 8:2023A molecular signature for IL-10-producing Th1 cells in protozoan parasitic diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChelsea L Edwards,Jessica A Engel,Fabian de Labastida Rivera,Susanna S Ng,Dillon Corvino,Marcela Montes de Oca,Teija Cm Frame,Shashi Bhushan Chauhan,Siddharth Sankar Singh,Awnish Kumar,Yulin Wang,Jinrui Na,Pam Mukhopadhyay,Jason S Lee,Susanne Nylen,Shyam Sundar,Rajiv Kumar,Christian R EngwerdaPubMed 37917177
- Cell biochemistry and function 41:1526-15422023An increase in intercellular crosstalk and electrotonic coupling between cardiomyocytes and nonmyocytes reshapes the electrical conduction in the metabolic heart characterized by short QT intervals in ECGs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeniz Billur,Yusuf Olgar,Aysegul Durak,Ayse Hande Yozgat,Simge Unay,Erkan Tuncay,Belma TuranPubMed 38014767
- Cell death & disease 14:7592023PDPN CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaoyin Li,Zeyi Ma,Weiyu Li,Xiaoqing Xu,Peiqi Shen,Si-En Zhang,Bin Cheng,Juan XiaPubMed 37993428
- Journal of experimental & clinical cancer research : CR 42:2702023UGDH promotes tumor-initiating cells and a fibroinflammatory tumor microenvironment in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittney S Harrington,Rahul Kamdar,Franklin Ning,Soumya Korrapati,Michael W Caminear,Lidia F Hernandez,Donna Butcher,Elijah F Edmondson,Nadia Traficante,Joy Hendley,Madeline Gough,Rebecca Rogers,Rohan Lourie,Jyoti Shetty,Bao Tran,Fathi Elloumi,Abdalla Abdelmaksoud,Madhu Lal Nag,Krystyna Mazan-Mamczarz,Carrie D House,John D Hooper,Christina M AnnunziataPubMed 37858159
- Oncology reports 50:2023Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhitao Chen,Peipei Li,Lingguang Shen,Xiuyu JiangPubMed 37732539
- Genome biology 24:1982023A DNA adenine demethylase impairs PRC2-mediated repression of genes marked by a specific chromatin signature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingxiao Jia,Xinran Zhang,Qian Liu,Junjie Li,Wentao Wang,Xuan Ma,Bo Zhu,Sheng Li,Shicheng Gong,Jingjing Tian,Meng Yuan,Yu Zhao,Dao-Xiu ZhouPubMed 37649077
- iScience 26:1076752023Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Hwan Kim,Seon Ju Mun,Jeong-Hwan Kim,Myung Jin Son,Seon-Young KimPubMed 37680467
- Cancer cell international 23:1652023ADAM8 is expressed widely in breast cancer and predicts poor outcome in hormone receptor positive, HER-2 negative patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefania Pianetti,Kathy D Miller,Hannah H Chen,Sandra Althouse,Sha Cao,Steven J Michael,Gail E Sonenshein,Nora D MinevaPubMed 37568162
- Nature communications 14:41932023ACTL6A protects gastric cancer cells against ferroptosis through induction of glutathione synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqing Yang,Shaomin Zou,Yijing Zhang,Jieping Zhang,Peng Zhang,Lishi Xiao,Yunling Xie,Manqi Meng,Junyan Feng,Liang Kang,Mong-Hong Lee,Lekun FangPubMed 37443154
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Constituents: 100% Borate buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Consider this alternative
Reactivity data
Select an application| IHC-P | ChIP/Chip | ICC/IF | ELISA | Flow Cyt | |
|---|---|---|---|---|---|
| Recombinant fragment - Human | Expected | Expected | Expected | Not recommended | Not recommended |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP/Chip
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
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Rabbit polyclonal IgG isotype control antibody that is used to detect IgG in IHC-P, ICC/IF, Flow cytometryometry, ChIP, ELISA.
- An isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions
- Cited in over 270 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Constituents: 100% Borate buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification
- Specificity
This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
- Concentration
- Purification notes
Protein A-purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Rabbit IgG, polyclonal - Isotype Control (ab37415) is validated for use in ChIP/Chip, ELISA, Flow Cyt, ICC/IF, IHC-P in cow, human samples.
Rabbit IgG, polyclonal - Isotype Control (ab37415) has been cited over 279 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Rabbit IgG, polyclonal - Isotype Control (ab37415) has high sensitivity and specificity. Rabbit IgG Isotype Control (ab37415) is sold in 5 mg selling sizes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)
Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.
PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.
Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)
ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.
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