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Rabbit IgG, polyclonal - Isotype Control Suitable for IHC-P, ChIP/Chip, ICC/IF, ELISA, Flow Cyt and reacts with Recombinant fragment - Human, samples. Cited in 278 publications.

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Images

Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (AB37415), expandable thumbnail
  • ChIP - Rabbit IgG, polyclonal - Isotype Control (AB37415), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 8.2
Constituents: 100% Borate buffered saline

Form

Liquid

Clonality

Polyclonal

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PChIP/ChipICC/IFELISAFlow Cyt
Recombinant fragment - Human
Expected
Expected
Expected
Not recommended
Not recommended

Expected
Expected

Species

Recombinant fragment - Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Recombinant fragment - Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Recombinant fragment - Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Rabbit IgG, polyclonal - Isotype Control Suitable for IHC-P, ChIP/Chip, ICC/IF, ELISA, Flow Cyt and reacts with Recombinant fragment - Human, samples. Cited in 278 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification

Specificity

This antibody has been selected to be an isotype control as it has no known specificity.

Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.

Concentration
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Purification notes

Protein A-purified

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

U+00A0;U+00A0;

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415), expandable thumbnail
    Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)

    Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.

    PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.

  • ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415), expandable thumbnail
    Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)

    ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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