Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) Suitable for WB, ChIP, Flow Cyt (Intra) and reacts with samples. Cited in 286 publications.
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- RNA biology 21:1-92023Multiple Oligo assisted RNA Pulldown via Hybridization followed by Mass Spectrometry (MORPH-MS) for exploring the RNA-Protein interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriyanka Pant,Regalla KumarswamyPubMed 38105541
- Regenerative therapy 25:35-482023Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinghui Xu,Zhenxiao Ren,Tianzuo Niu,Siyuan LiPubMed 38058606
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e232882023LINC02454 promotes thyroid carcinoma progression via upregulating HMGA2 through CREB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Cao,Jian Li,Yongliang Du,Yuxuan Sun,Le Liu,Hao Fang,Yan Liang,Shanshan MaoPubMed 37997502
- International journal of oncology 63:2023MEK/ERK and PI3K/AKT pathway inhibitors affect the transformation of myelodysplastic syndrome into acute myeloid leukemia via H3K27me3 methylases and de‑methylases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuanzhen Zheng,Xiuhua Chen,Yaofang Zhang,Fanggang Ren,Yanping MaPubMed 37921060
- Cell communication and signaling : CCS 21:2942023Transgenerational inheritance of adrenal steroidogenesis inhibition induced by prenatal dexamethasone exposure and its intrauterine mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng He,Jinzhi Zhang,Yawen Chen,Can Ai,Xiaohan Gong,Dan Xu,Hui WangPubMed 37853416
- MedComm 4:e3842023Differential traits between microvesicles and exosomes in enterovirus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxuan Fu,Sidong XiongPubMed 37752943
- The FEBS journal 290:5395-54102023Neutrophil extracellular traps boost laser-induced mouse choroidal neovascularization through the activation of the choroidal endothelial cell TLR4/HIF-1α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zeng,Ying Wang,Manhui Zhu,Min Wu,Yamei Zhou,Qiaoyun Wang,Yiqian Xu,Fei Lin,Jiaqi Wang,Yuxuan Li,Shuang Liang,Ziyu Wang,Laiqing Xie,Xiaojuan LiuPubMed 37552110
- Anatomia, histologia, embryologia 52:874-8812023Immunolocalization and expression of Siglec5 protein in the male reproductive tract of dromedary camel during rutting season.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalid Mohamed Al Khodair,Mohammed Salem Moqbel,Abdelrahman Mohamed Ali Elseory,Mahmoud Gamil Elsebaei,Thnaian A Al-Thnaian,Mortada M O ElhassanPubMed 37431856
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229592023TFAP2C inhibits cell autophagy to alleviate myocardial ischemia/reperfusion injury by regulating miR-23a-5p/SFRP5/Wnt5a axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Zeng,Xin Wei,Yang-Li He,Ji-Xiong Chen,Wen-Ting LinPubMed 37191968
- Cell reports 42:1124712023The glycolysis/HIF-1α axis defines the inflammatory role of IL-4-primed macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBuyun Dang,Qingxiang Gao,Lishan Zhang,Jia Zhang,Hanyi Cai,Yanhui Zhu,Qiumei Zhong,Junqiao Liu,Yujia Niu,Kairui Mao,Nengming Xiao,Wen-Hsien Liu,Shu-Hai Lin,Jialiang Huang,Stanley Ching-Cheng Huang,Ping-Chih Ho,Shih-Chin ChengPubMed 37149865
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
Application ChIP | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
Application Flow Cyt (Intra) | Reactivity Reacts | Dilution info - | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
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Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) Suitable for WB, ChIP, Flow Cyt (Intra) and reacts with samples. Cited in 286 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Purification technique
Affinity purification Protein G
- Specificity
The sera for this product is un-immunized, naive sera.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
For more information regarding the isotype control selection, please see https://www.abcam.com/primary-antibodies/your-guide-to-selecting-an-isotype-control
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Flow Cytometry (Intracellular) - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Overlay histogram showing HeLa cells stained with ab75186 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75186, 0.05μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/4000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.05μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Please note that ab171870 in lanes 1-6 represents a negative control for Beta Actin, positively seen in lanes 7-12.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) at 1 µg/mL
Lanes 1 and 7: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lanes 2 and 8: Liver (Mouse) Tissue Lysate at 10 µg
Lanes 3 and 9: Liver (Rat) Tissue Lysate at 10 µg
Lanes 10 and 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 11 and 5: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lanes 12 and 6: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2min
Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab171870 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate - adult normal tissue at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: Rat Kidney Tissue Lysate at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20min
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