Name: Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein
SKU: ab271180
Rating: 5 (4 reviews)

Rabbit Recombinant Monoclonal NCAP antibody. Suitable for ICC/IF, IP, ELISA, WB, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - SARS-CoV-2 samples. Cited in 6 publications.

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Images

Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (AB271180), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	ELISA	WB	Flow Cyt (Intra)	IHC-P
Recombinant fragment - SARS-CoV-2	Tested	Tested	Tested	Tested	Tested	Tested
SARS-CoV-2	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info 1/30	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info -	Notes Use at 31.25 ng /ml

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info 1/500	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - SARS-CoV-2	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info -	Notes -

Target data

See full target information N

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Alternative names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

Recommended products

Rabbit Recombinant Monoclonal NCAP antibody. Suitable for ICC/IF, IP, ELISA, WB, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - SARS-CoV-2 samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24334-118
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This antibody is developed with the collaboration of Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

Abcam offers a variety of tools used to help understand and accelerate infectious disease research, including SARS-CoV-2, the coronavirus that causes COVID-19.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

Associated diseases and disorders

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time 7.75 seconds
All lanes: Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: HEK-293T (human embryonic kidney) transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 3: HEK-293T (human embryonic kidney) transfected with MERS Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa
Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.
All lanes: Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution
Lane 1: SARS-CoV-2 Nucleocapsid Recombinant Protein at 0.1 µg
Lane 2: SARS-CoV-2 infected Vero for 48 hours
Lane 3: Uninfected Vero
Observed band size: 51 kDa
Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Lanes 1 - 3: Green - ab271180 observed at 51 kDa. Lanes 4 - 6: Red - loading control Anti-6X His tag® antibody [HIS.H8] ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag^®) observed at 51 kDa.
ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab271180 and Anti-6X His tag® antibody [HIS.H8] ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag^®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180) at 1/1000 dilution
Lanes 1 and 4: Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) at 0.1 µg
Lanes 2 and 5: Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.1 µg
Lanes 3 and 6: Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) ab272107) at 0.1 µg
Performed under reducing conditions.
Observed band size: 51 kDa
Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Samples
Lanes 1 & 3: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) (0.2 μg)
Lanes 2 & 4: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) ab272107) (0.2 μg)
Green: Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Red: Mouse monoclonal [HIS.H8] to 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184)
Lanes 1 & 2: Green - ab271180 observed at 50 kDa.
Lanes 3 & 4: Red - loading control Mouse anti-6x HisTag observed at 50 kDa.
ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag (Anti-6X His tag® antibody [HIS.H8] ab18184) overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
All lanes: Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530)
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunohistochemical analysis of paraffin-embedded Human lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on human lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel A)
HEK-293T transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel B) HEK-293T transfected with MERS Nucleocapsid protein expression vector containing a myc-His-tag® (Panel C) HEK-293T transfected with control vector containing a myc-His-tag® (Panel D) with 271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on (A) HEK-293T transfected with a SARS-CoV-2 Nucleocapsid protein expression vector is observed. No staining on (B) HEK-293T transfected with a SARS-CoV-1 Nucleocapsid protein expression vector, (C) HEK-293T transfected with MERS Nucleocapsid protein expression vector and (D) HEK-293T transfected with empty vector. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunohistochemical analysis of paraffin-embedded Mouse lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Mouse lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunohistochemical analysis of paraffin-embedded Rat lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Rat lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Alexa Fluor® 488 Anti-Myc tag antibody [9E10] ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector.
Immunoprecipitation - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
SARS-CoV-2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate 5 μg with ab271180 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate 5 μg
Lane 2: ab271180 IP in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271180 in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
All lanes: Immunoprecipitation - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Observed band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized Vero cells labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/500 dilution. Fluorescent image showing positve staining in Vero cells after infecting with SARS-CoV-2 virus for 48 h. Nuclear counter stain DAPI.
Two indenpendent optical fields were captured for imaging at each given antibody dilution.
This piece of data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine
Immunocytochemistry/ Immunofluorescence - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Alexa Fluor® 488 Anti-Myc tag antibody [9E10] ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing no staining in 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein, which are negative controls for SARS-CoV-2.
Flow Cytometry (Intracellular) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression construct labelling SARS-CoV-2 with ab271180 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) isotype control.
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-1 Nucleocapsid protein expression construct (Left)/ HEK-293T (human embryonic kidney) transfected with myc-tagged MERS Nucleocapsid protein expression construct (Right).
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/2000 dilution was used as the secondary antibody.
ELISA - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.
Primary antibody (ab271180) concentration range 0.045-100 ng /ml.
Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) used at 1/2000 dilution.