Anti-GAD67/GAD1

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 (AB97739)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling GAD67 protein using ab97739 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 (AB97739)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for GAD67 protein at cytoplasm with ab97739 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 (AB97739)

Immunofluorescence analysis of rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling GAD67 protein using ab97739 at a 1/500 dilution (Green). Tau, stained by an anti-Tau antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 (AB97739)

Immunohistochemical analysis of paraffin-embedded mouse eye tissue stained for GAD67 protein at cell membrane and cytoplasm with ab97739 at a 1/250 dilution (Green). Tubulin 3/ Tuj1, a neural marker, stained by a beta Tubulin 3/ Tuj1 antibody diluted at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 (AB97739)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue stained for GAD67 protein at cell membrane and cytoplasm with ab97739 at a 1/1000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 (AB97739)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue stained for GAD67 protein at cell membrane and cytoplasm with ab97739 at a 1/1000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 (AB97739)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for GAD67 protein at cytoplasm with ab97739 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 (AB97739)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GAD67/GAD1 (ab97739) at 1/1000 dilution

All lanes:

Human tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 (AB97739)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GAD67/GAD1 (ab97739) at 1/500 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 (AB97739)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GAD67/GAD1 (ab97739) at 1/5000 dilution

Lane 1:

Wild type 293T whole cell lysate at 30 µg

Lane 2:

Human GAD1-transfected 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 (AB97739)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GAD67/GAD1 (ab97739) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 67 kDa

false