Rabbit Polyclonal RABEP1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RABEP1 aa 800 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RABEP1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15276
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane (PubMed:22841712). Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (By similarity).
Alternative names
RAB5EP, RABPT5, RABPT5A, RABEP1, Rab GTPase-binding effector protein 1, Rabaptin-4, Rabaptin-5, Rabaptin-5alpha, Renal carcinoma antigen NY-REN-17
Recommended products
Rabbit Polyclonal RABEP1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human RABEP1 aa 800 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RABEP1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15276
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176578 was affinity purified using an epitope specific to RABEP1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RABEP1 also known as Rabaptin-5 is a protein involved in membrane trafficking processes and it has a molecular mass of approximately 101 kDa. It acts as a linker connecting Rab5 and other effector proteins. RABEP1 operates predominantly within the early endosomes where it plays a part in the regulation of endocytic pathways. The protein exhibits expression in various tissues although more investigation may be necessary to fully map its exact distribution.
- Biological function summary
RABEP1 functions as a central component in early endosome fusion and trafficking processes. It often forms part of a complex with the Rab5 protein facilitating the tethering and docking of vesicles within the intracellular environment. Through its interactions it plays roles in processes such as endocytosis and recycling of membrane receptors. The RABEP1's activities ensure proper sorting and transport of cargo molecules contributing to cellular homeostasis.
- Pathways
RABEP1 contributes significantly to both the endocytic and recycling pathways. It associates closely with the Rab family of GTPases particularly Rab5 during the early stages of endosomal transport. This protein helps in transitioning cargo through the endosomes an important part of the Rab5-related endocytic pathway. Also RABEP1 works collaboratively with the EEA1 protein assisting in the regulation of early endosome membrane fusion and cargo sorting.
- Associated diseases and disorders
RABEP1 shows associations with neurodegenerative and metabolic conditions. Studies link its dysfunction with Alzheimer's disease implicating its connection with tau protein aggregation. RABEP1 also reveals relationships with the insulin receptor through endosomal trafficking pathways indicating potential involvement in metabolic disorders like type 2 diabetes. Understanding these connections may open avenues for therapeutic interventions targeting these pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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2 product images
Western blot - Anti-RABEP1 antibody (ab176578)
All lanes: Western blot - Anti-RABEP1 antibody (ab176578) at 0.04 µg/mL
Lane 1: HeLa whole cell lysates at 50 µg
Lane 2: HeLa whole cell lysates at 15 µg
Lane 3: HeLa whole cell lysates at 5 µg
Lane 4: 293T whole cell lysates at 50 µg
Lane 5: NIH3T3 whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 30s
Immunoprecipitation - Anti-RABEP1 antibody (ab176578)
Detection of RABEP1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176578 at 3 μg/mg lysate for IP (Lane 1). For WB detection ab176578 antibody which recognizes an upstream epitope was used at 0.4 μg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-RABEP1 antibody (ab176578)
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3s
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