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AB190003

Anti-RABEP2 antibody

1

(1 Review)

|

(1 Publication)

Rabbit Polyclonal RABEP2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RABEP2 aa 350-450.

View Alternative Names

RABPT5B, RABEP2, Rab GTPase-binding effector protein 2, Rabaptin-5beta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABEP2 antibody (AB190003)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABEP2 antibody (AB190003)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon sections labeling RABEP2 with ab190003 at 10 μg/ml.

Western blot - Anti-RABEP2 antibody (AB190003)
  • WB

Supplier Data

Western blot - Anti-RABEP2 antibody (AB190003)

All lanes:

Western blot - Anti-RABEP2 antibody (ab190003) at 1/500 dilution

Lane 1:

Extracts from HeLa cells

Lane 2:

Extracts from Jurkat cells

Lane 3:

Extracts from 293 cells

Lane 4:

Extracts from HUVEC cells

Lane 5:

Extracts from HeLa cells with synthesized peptide

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RABEP2 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H5N1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RABEP2 also known as Rabaptin-5-like protein or Rabaptin-5b is a protein involved in endosomal trafficking. It has a molecular weight of approximately 50 kDa. RABEP2 functions as an effector protein for Rab GTPases which are critical regulators of membrane traffic in the cell. This protein is widely expressed in various tissues playing key roles in intracellular transport processes. RABEP2 associates with particular endosomal membranes facilitating vesicle budding movement and fusion.
Biological function summary

RABEP2 facilitates membrane trafficking by working in the context of endosomal membrane fusion. It forms part of a larger protein complex that interacts with Rab GTPases especially Rab5 which is a well-known regulator of early endosome dynamics. By binding these GTPases RABEP2 stabilizes active Rab GTPase configurations assisting in their recruitment to endosomal membranes. This mechanism is essential for efficient transport and sorting of internalized molecules.

Pathways

RABEP2 plays a role in cellular pathways involving receptor-mediated endocytosis and vesicle trafficking. In these pathways it connects with the early endosome and sorting endosome pathways which are vital for recycling and degradation processes. RABEP2 works closely with other Rab family proteins like Rab4 and Rab11 sharing roles in ensuring proper receptor allocation and signal transduction. This cooperation enhances the cell's ability to adaptively respond to changes in the extracellular environment.

Modifications or dysfunctions in RABEP2 have associations with cancer and neurodegenerative diseases. Disruptions in Rab GTPase networks including alterations involving RABEP2 can lead to dysfunctional endosomal trafficking which contributes to tumor progression by affecting growth factor receptor recycling. Relationships exist between RABEP2 functionality and other proteins like Rab5 where altered interactions may result in neurodegenerative conditions due to the cumulative effects on synaptic function and neuronal survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in membrane trafficking and in homotypic early endosome fusion (PubMed : 9524116). Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking (PubMed : 29425100). By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis (PubMed : 27224062).
See full target information RABEP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:4805-4817 PubMed29425100

2018

The Rab-effector protein RABEP2 regulates endosomal trafficking to mediate vascular endothelial growth factor receptor-2 (VEGFR2)-dependent signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Kofler,Federico Corti,Felix Rivera-Molina,Yong Deng,Derek Toomre,Michael Simons
View all publications
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Product promise

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