Rabbit Polyclonal RABGAP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus.
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Publications
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- Journal of cell science 133:2020Rabgap1 promotes recycling of active β1 integrins to support effective cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna V Samarelli et. al.PubMed 32843574
- Cell discovery 4:12018TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatsumi Kawasaki et. al.PubMed 29423269
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3P9
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
Alternative names
HSPC094, RABGAP1, Rab GTPase-activating protein 1, GAP and centrosome-associated protein, Rab6 GTPase-activating protein GAPCenA
Recommended products
Rabbit Polyclonal RABGAP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3P9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RABGAP1 also known as Rab GTPase-activating protein 1 plays a significant role in regulating the activity of Rab proteins by promoting GTP hydrolysis to GDP. This regulation alters the Rab's affinity for membranes and their associated regulators and effectors. RABGAP1 has a mass of approximately 77 kDa and is functionally expressed in a range of tissues including those of the brain and pancreas indicating its importance in various cellular processes.
- Biological function summary
RABGAP1 interacts with cellular components to modulate vesicular trafficking and membrane dynamics. It acts as a negative regulator in processes involving endocytosis and secretion by interfering in the Rab GTPase signaling cycle. This protein is not necessarily part of a larger multiprotein complex but works closely with members of the Rab family proteins. Through its activity RABGAP1 ensures accuracy in the transportation and sorting of cellular materials.
- Pathways
RABGAP1 is involved in the regulation of the intracellular trafficking pathways particularly those linked with endocytosis and secretory routes. It functions in pathways associated with Rab GTPases influencing cellular processes such as vesicle budding and transport. This involvement places RABGAP1 in close functional association with proteins like Rab1 and Rab5 which are essential for membrane trafficking events and maintaining cellular organization.
- Associated diseases and disorders
Dysfunction of RABGAP1 is associated with alterations in cellular transport mechanisms which could contribute to diseases such as cancer and neurological disorders. Aberrant activity or expression levels of RABGAP1 may lead to the misregulation of protein trafficking therefore influencing cancer metastasis or neuronal pathologies. It may interact with other proteins related to these disorders such as Rab11 highlighting its role in the broader context of disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992)
7.5% SDS PAGE
All lanes: Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/10000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 121 kDa
Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992)
7.5% SDS PAGE
All lanes: Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/5000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 121 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1 antibody - C-terminal (ab153992)
Immunohistochemical analysis of paraffin-embedded BT474
xenograft labeling RABGAP1 with ab153992 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RABGAP1 antibody - C-terminal (ab153992)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling RABGAP1 with ab153992 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
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