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AB153992

Anti-RABGAP1 antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal RABGAP1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus.

View Alternative Names

HSPC094, RABGAP1, Rab GTPase-activating protein 1, GAP and centrosome-associated protein, Rab6 GTPase-activating protein GAPCenA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1 antibody - C-terminal (AB153992)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1 antibody - C-terminal (AB153992)

Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling RABGAP1 with ab153992 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RABGAP1 antibody - C-terminal (AB153992)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RABGAP1 antibody - C-terminal (AB153992)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling RABGAP1 with ab153992 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.

Western blot - Anti-RABGAP1 antibody - C-terminal (AB153992)
  • WB

Unknown

Western blot - Anti-RABGAP1 antibody - C-terminal (AB153992)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/10000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 121 kDa

false

Western blot - Anti-RABGAP1 antibody - C-terminal (AB153992)
  • WB

Unknown

Western blot - Anti-RABGAP1 antibody - C-terminal (AB153992)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/5000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RABGAP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3P9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RABGAP1 also known as Rab GTPase-activating protein 1 plays a significant role in regulating the activity of Rab proteins by promoting GTP hydrolysis to GDP. This regulation alters the Rab's affinity for membranes and their associated regulators and effectors. RABGAP1 has a mass of approximately 77 kDa and is functionally expressed in a range of tissues including those of the brain and pancreas indicating its importance in various cellular processes.
Biological function summary

RABGAP1 interacts with cellular components to modulate vesicular trafficking and membrane dynamics. It acts as a negative regulator in processes involving endocytosis and secretion by interfering in the Rab GTPase signaling cycle. This protein is not necessarily part of a larger multiprotein complex but works closely with members of the Rab family proteins. Through its activity RABGAP1 ensures accuracy in the transportation and sorting of cellular materials.

Pathways

RABGAP1 is involved in the regulation of the intracellular trafficking pathways particularly those linked with endocytosis and secretory routes. It functions in pathways associated with Rab GTPases influencing cellular processes such as vesicle budding and transport. This involvement places RABGAP1 in close functional association with proteins like Rab1 and Rab5 which are essential for membrane trafficking events and maintaining cellular organization.

Dysfunction of RABGAP1 is associated with alterations in cellular transport mechanisms which could contribute to diseases such as cancer and neurological disorders. Aberrant activity or expression levels of RABGAP1 may lead to the misregulation of protein trafficking therefore influencing cancer metastasis or neuronal pathologies. It may interact with other proteins related to these disorders such as Rab11 highlighting its role in the broader context of disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
See full target information RABGAP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 133: PubMed32843574

2020

Rabgap1 promotes recycling of active β1 integrins to support effective cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Anna V Samarelli,Tilman Ziegler,Alexander Meves,Reinhard Fässler,Ralph T Böttcher

Cell discovery 4:1 PubMed29423269

2018

TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Natsumi Kawasaki,Kazunobu Isogaya,Shingo Dan,Takao Yamori,Hiroshi Takano,Ryoji Yao,Yasuyuki Morishita,Luna Taguchi,Masato Morikawa,Carl-Henrik Heldin,Tetsuo Noda,Shogo Ehata,Kohei Miyazono,Daizo Koinuma
View all publications
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Product promise

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