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AB229956

Anti-RABGAP1L/HHL antibody

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(1 Publication)

Rabbit Polyclonal RBG10 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RABGAP1L aa 1-300.

View Alternative Names

HHL, KIAA0471, RABGAP1L

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1L/HHL antibody (AB229956)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1L/HHL antibody (AB229956)

Paraffin-embedded human gastric cancer tissue stained for RABGAP1L/HHL using ab229956 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1L/HHL antibody (AB229956)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABGAP1L/HHL antibody (AB229956)

Paraffin-embedded human liver cancer tissue stained for RABGAP1L/HHL using ab229956 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-RABGAP1L/HHL antibody (AB229956)
  • WB

Supplier Data

Western blot - Anti-RABGAP1L/HHL antibody (AB229956)

All lanes:

Western blot - Anti-RABGAP1L/HHL antibody (ab229956) at 1/2000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

Mouse stomach lysate

Lane 5:

Mouse heart lysate

Lane 6:

Mouse brain lysate

Lane 7:

Mouse liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 93 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RABGAP1L aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

B7ZAP0

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA2 antibody (AB254590)

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGEF1A antibody (AB229272)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RABGAP1L also known as HHL is a GTPase-activating protein (GAP) that targets Rab GTPases which are important regulators of intracellular vesicle transport. The exact mass of the RABGAP1L protein is not well-documented but it is expressed in various tissues with significant presence in brain among others. This distribution suggests RABGAP1L's involvement in diverse cellular processes especially those requiring active vesicle trafficking and membrane dynamics.
Biological function summary

This protein acts as a regulatory molecule modulating the activity of Rab GTPases by accelerating the hydrolysis of GTP to GDP. This action helps switch the GTPases from an active to an inactive state which is vital for the spatial and temporal regulation of vesicular trafficking. RABGAP1L frequently associates with multiprotein complexes contributing to its regulatory functions in trafficking and signaling pathways.

Pathways

The protein plays a significant role in the endocytosis and exocytosis pathways that are vital for recycling membrane and macromolecules. RABGAP1L interacts with proteins like Rab11 in the endosomal recycling compartment which is essential for the efficient sorting and recycling of transmembrane proteins. These interactions highlight its involvement in the cellular management of vesicular transport and suggest its regulatory influence on several cellular processes.

RABGAP1L links to conditions such as neurological disorders and cancer where dysregulation of vesicular trafficking is a common factor. For instance its interaction with proteins involved in synaptic vesicle trafficking possibly associates it with certain neurodegenerative disorders. It also relates with oncogenic processes through its influence on the transport pathways mirroring dysfunctions seen in other regulatory proteins involved in cancer pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information RABGAP1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113311 PubMed40917882

2025

An iPSC-derived neuronal model reveals manganese's role in neuronal endocytosis, calcium flux and mitochondrial bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitri Budinger,Sharmin Alhaque,Ramón González-Méndez,Chris Dadswell,Katy Barwick,Arianna Ferrini,Charlotte Roth,Conor J McCann,Karin Tuschl,Fatma Al Jasmi,Maha S Zaki,Julien H Park,Russell C Dale,Shekeeb Mohammad,John Christodoulou,Dale Moulding,Michael R Duchen,Serena Barral,Manju A Kurian
View all publications
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Product promise

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