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AB205029

Anti-RABIF antibody

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(1 Publication)

Rabbit Polyclonal RABIF antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RABIF aa 1 to C-terminus.

View Alternative Names

MSS4, RASGRF3, RABIF, Guanine nucleotide exchange factor MSS4, Rab-interacting factor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABIF antibody (AB205029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABIF antibody (AB205029)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human fetal colon tissue labeling RABIF with ab205029 at a 1/100 dilution.

Western blot - Anti-RABIF antibody (AB205029)
  • WB

Supplier Data

Western blot - Anti-RABIF antibody (AB205029)

All lanes:

Western blot - Anti-RABIF antibody (ab205029) at 1/500 dilution

Lane 1:

HL60 cell lysate

Lane 2:

HL29 cell lysate

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RABIF aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47224

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RABIF also known as Ras-related protein Rab guanine nucleotide exchange factor functions as a regulatory protein that facilitates the exchange of GDP for GTP on Rab proteins a process essential for their activation. The weight of RABIF is approximately 56 kDa. It finds expression in various tissues with significant presence in the pancreas and the brain. RABIF operates in intracellular trafficking by regulating dynamics of Rab GTPases which are important for vesicular transport mechanisms within cells.
Biological function summary

RABIF influences membrane trafficking processes by aiding the activation of Rab proteins which are part of a larger Rab family involved in vesicle movement between organelles. The protein functions as part of a larger complex contributing to the precise targeting and fusion of vesicles. This role is particularly important for maintaining cellular organization and homeostasis as it coordinates the movement of proteins and lipids within cells.

Pathways

Regulation by RABIF is essential in vesicular transport pathways such as endocytosis and exocytosis. Its activity is important for the actin cytoskeleton dynamics through interactions with proteins like Rab8 and Rab10 which affect cell shape and mobility. RABIF's functions align with pathways that manage intercellular signaling and membrane dynamics facilitating the transport essential for cellular responses to environmental changes.

Dysregulation of RABIF is linked with disorders like cancer and neurodegenerative diseases where vesicle transport and cell signaling play an important role. The protein interacts with factors like Rab3 which are significant for neurotransmitter release in neurons hinting at its implication in synaptic dysfunction present in conditions like Alzheimer's disease. Understanding RABIF’s function can provide insight into therapeutic targets for these complex diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine-nucleotide-releasing protein that acts on members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1, RAB3A and RAB10, but is less active on these proteins than on the SEC4 protein (PubMed : 31540829). Might play a general role in vesicular transport.
See full target information RABIF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:237 PubMed35301428

2022

KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ru Cui,Shao-Jie Wang,Tiancheng Ma,Peihong Yu,Jun Chen,Taijie Guo,Genyi Meng,Biao Jiang,Jiajia Dong,Jia Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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