Anti-Rabphilin 3A antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (AB3338)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase RSC96 (rat neuronal Schwann cell) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling Rabphilin 3A with ab3338 at 2 μg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Rabphilin 3A antibody (AB3338)

Detection : chemiluminescence

Rabphilin 3A observed in Mouse and Rat Brain, and not observed in other tissues which are documented to be Rabphilin 3A negative.

All lanes:

Western blot - Anti-Rabphilin 3A antibody (ab3338) at 3 µg/mL

Lane 1:

Mouse Brain at 30 µg

Lane 2:

Rat Brain at 30 µg

Lane 3:

Rat Lung at 30 µg

Lane 4:

Rat Liver at 30 µg

Lane 5:

Rat Kidney at 30 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG at 1/4000 dilution

Predicted band size: 77 kDa

Observed band size: ~76 kDa

false

• WB

Supplier Data

Western blot - Anti-Rabphilin 3A antibody (AB3338)

Detection : chemiluminescence

All lanes:

Western blot - Anti-Rabphilin 3A antibody (ab3338) at 2 µg/mL

All lanes:

Rat Brain

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution

Predicted band size: 77 kDa

Observed band size: ~76 kDa

false

• WB

Unknown

Western blot - Anti-Rabphilin 3A antibody (AB3338)

Western blot detection of Rabphilin 3A in rat brain extract using ab3338.

All lanes:

Western blot - Anti-Rabphilin 3A antibody (ab3338)

Predicted band size: 77 kDa

false