AB247485

Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CDC42 phospho S71 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42

1 Images

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Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] - BSA and Azide free (AB247485)

This data was developed using ab76535, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (<a href='/en-us/products/primary-antibodies/rac1-cdc42-phospho-s71-antibody-ep221y-ab76535'>ab76535</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate treated with Lambda Phosphatase at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 24 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP221Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247485 is the carrier-free version of ab76535.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAC1 and Cdc42 are small Rho GTPases involved in regulating various cellular processes related to the cytoskeleton. RAC1 also known as Ras-related C3 botulinum toxin substrate 1 has a molecular mass of approximately 21 kDa. These proteins are found in a wide range of tissues displaying high expression levels in places like the brain and immune cells. They function as molecular switches cycling between an active GTP-bound state and an inactive GDP-bound state facilitating signal transduction for numerous cellular responses.

Biological function summary

RAC1 and Cdc42 critically influence actin cytoskeleton dynamics and cell morphology. They are part of the Rho family small GTPase interactive with downstream effectors to control cell shape movement and division. These proteins play significant roles in membrane trafficking cell cycle progression and apoptosis. RAC1 along with Cdc42 forms part of a complex network that is essential for functional cytoskeleton reorganization important in neuronal activity and immune cell functioning.

Pathways

RAC1 and Cdc42 mediate signal transduction in the PI3K/AKT pathway and the Wnt signaling pathway. In the PI3K/AKT pathway these proteins interact with other components like PAK proteins which serve as essential mediators for survival and growth signals. In the Wnt pathway RAC1 and Cdc42 influence gene transcription helping control cellular proliferation and differentiation. Their roles in these pathways show their importance in maintaining cellular homeostasis.

RAC1 and Cdc42 connect to cancer and neurological disorders. Alterations in their activity can contribute to tumor growth and metastasis as their dysregulation affects cell migration and invasion. RAC1 is especially noted for its role in various cancer types. Additionally their imbalance may lead to neurological disorders due to their effect on nerve growth and branching. RAC1 and Cdc42's interactions with proteins like RhoA and β-catenin underline their significance in pathophysiological conditions making them critical targets for therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed : 15642749). Regulates cell migration (PubMed : 17038317, PubMed : 22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed : 14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed : 33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed : 26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed : 33310911).

See full target information Cell division control protein 42 homolog phospho S71

Additional targets

RAC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] - BSA and Azide free

Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] - BSA and Azide free (AB247485)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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Primary Antibodies

Primary Antibodies

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