Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631]

• WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (AB180683)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time :

Lanes 1 & 2 : 1 second.

Lane 3 : 3 minutes.

Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac's were made in house.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

Lane 1:

Recombinant Human Rac1 full length protein at 0.01 µg

Lane 2:

Recombinant Human Rac2 full length protein at 0.01 µg

Lane 3:

Recombinant Human Rac3 full length protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (AB180683)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 seconds; Lane 2-4 : 15 seconds.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (AB180683)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (AB180683)

Blocking and diluting buffer : 5% NFDM/TBST. Exposure time lanes 1-3 : 7 seconds, lanes 4-6 : 40 seconds.

Lanes 1 - 3:

Western blot - Anti-RAC3 antibody [EPR6680] (<a href='/en-us/products/primary-antibodies/rac3-antibody-epr6680-ab129062'>ab129062</a>) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/1000 dilution

Lanes 1 and 4:

C-Myc/DDK tagged Human full length RAC1 recombinant protein at 10 ng

Lanes 2 and 5:

His-tagged Human full length RAC2 recombinant protein at 10 ng

Lanes 3 and 6:

C-Myc/DDK tagged Human full length RAC3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,25 kDa

false

• WB

CiteAb

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (AB180683)

Rac1 + Rac2 + Rac3 western blot using anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683. Publication image and figure legend from Chen, Y., Liu, Q., et al., 2020, Front Pharmacol, PubMed 31920663.

ab180683 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180683 please see the product overview.

Effects of catalpol on the activities of RhoA, Cdc42, and Rac1 in podocytes exposed to high glucose. (A) Active GTP-bound forms of RhoA, Cdc42, and Rac1 purified from podocytes cultured with NG (5.5 mmol/L), HG (40 mmol/L), and HG plus different concentrations of catalpol (1, 5, 10 μM) by GST pull-down assays and subjected to western blot (n = 3). (B) Quantification of results in panel A. Data represent the mean values ± SD from three independent experiments, **p < 0.01 vs NG, ##p < 0.01 vs HG.

false