Skip to main content

Rabbit Recombinant Monoclonal RAC1 phospho S71 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247), expandable thumbnail
  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247), expandable thumbnail
  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247), expandable thumbnail
  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Recombinant full length protein - Human
Tested

Tested
Tested

Species
Recombinant full length protein - Human, Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles (PubMed:1643658, PubMed:22843693, PubMed:23512198, PubMed:28886345). Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity (PubMed:9121475). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:1643658). In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity (By similarity). In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX (PubMed:12695502). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization (By similarity). Required for DSG3 translocation to cell-cell junctions, DSG3-mediated organization of cortical F-actin bundles and anchoring of actin at cell junctions; via interaction with DSG3 (PubMed:22796473). Isoform B. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins (PubMed:14625275). It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction (PubMed:14625275).

Additional Targets

RAC2, RAC3

Alternative names

TC25, MIG5, RAC1, Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1

Recommended products

Rabbit Recombinant Monoclonal RAC1 phospho S71 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18631
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250247 is the carrier-free version of Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rac1 Rac2 and Rac3 are small GTPases belonging to the Rho family of proteins often referred to as Rac GTPases. These proteins are involved mechanically in the regulation of cell growth cytoskeletal reorganization and the generation of reactive oxygen species. Rac1 has a molecular mass of approximately 21 kDa and Rac proteins share a highly conserved amino acid sequence. Rac1 is ubiquitously expressed while Rac2 is mostly found in hematopoietic cells and Rac3 is expressed in the brain and other tissues.

Biological function summary

Rac proteins play key roles in cell movement adhesion and proliferation. Rac1 Rac2 and Rac3 participate in the formation of lamellipodia which are cellular protrusions important for migration. These proteins are part of complexes involved in actin cytoskeleton dynamics affecting how cells interact with their environment. Different Rac isoforms contribute to the precise control and timing of cellular responses to external stimuli influencing processes such as immune cell function and neuronal development.

Pathways

The Rac proteins integrate into the signaling processes that control actin organization and cell cycle progression. The Rac GTPases interact with pathways such as the PI3K/Akt and MAPK pathways. In these pathways they partner with proteins like PAK (p21-activated kinase) to facilitate changes in the cytoskeleton and promote survival signals. These interactions allow cells to modify their shape and migrate in response to different stimuli enabling a connection between cell signaling and movement.

Associated diseases and disorders

Rac proteins link to several pathological conditions including cancer and immune-related disorders. Rac1 is often implicated in cancer where its activity affects tumor growth and metastasis through alterations in the cell cycle and motility. Rac2 mutations can lead to immune system dysfunctions impacting immune cell migration and response contributing to conditions like chronic granulomatous disease. The dysregulation of Rac activity often involves interactions with other protein partners like Nox enzymes which are responsible for reactive oxygen species production playing a part in disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247), expandable thumbnail

    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)

    This data was developed using Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 1 second; Lane 3: 3 minutes.

    Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac's were made in house.

    All lanes: Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683) at 1/2000 dilution

    Lane 1: Recombinant Human Rac1 full length protein at 0.01 µg

    Lane 2: Recombinant Human Rac2 full length protein at 0.01 µg

    Lane 3: Recombinant Human Rac3 full length protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247), expandable thumbnail

    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)

    This data was developed using Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lanes 2-4 : 15 seconds.

    All lanes: Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247), expandable thumbnail

    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)

    This data was developed using Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683) at 1/2000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

    Exposure time: 3s

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247), expandable thumbnail

    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683)

    Blocking and diluting buffer: 5% NFDM/TBST. Exposure time lanes 1-3: 7 seconds, lanes 4-6: 40 seconds.

    Lanes 1 - 3: Western blot - Anti-RAC3 antibody [EPR6680] (Anti-RAC3 antibody [EPR6680] ab129062) at 1/1000 dilution

    Lanes 4 - 6: Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683) at 1/1000 dilution

    Lanes 1 and 4: C-Myc/DDK tagged Human full length RAC1 recombinant protein at 10 ng

    Lanes 2 and 5: His-tagged Human full length RAC2 recombinant protein at 10 ng

    Lanes 3 and 6: C-Myc/DDK tagged Human full length RAC3 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 25 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com