JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250247

Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free

4

(2 Reviews)

|

(1 Publication)

Rabbit Recombinant Monoclonal Rac1 + Rac2 + Rac3 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

TC25, MIG5, RAC1, Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1

4 Images
Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)
  • WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)

This data was developed using ab180683, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 1 second; Lane 3 : 3 minutes.

Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac's were made in house.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (<a href='/en-us/products/primary-antibodies/rac1-rac2-rac3-antibody-epr18631-ab180683'>ab180683</a>) at 1/2000 dilution

Lane 1:

Recombinant Human Rac1 full length protein at 0.01 µg

Lane 2:

Recombinant Human Rac2 full length protein at 0.01 µg

Lane 3:

Recombinant Human Rac3 full length protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)
  • WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)

This data was developed using ab180683, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 seconds; Lanes 2-4 : 15 seconds.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (<a href='/en-us/products/primary-antibodies/rac1-rac2-rac3-antibody-epr18631-ab180683'>ab180683</a>) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)
  • WB

Supplier Data

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)

This data was developed using ab180683, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (<a href='/en-us/products/primary-antibodies/rac1-rac2-rac3-antibody-epr18631-ab180683'>ab180683</a>) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 3s

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)
  • WB

Lab

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (AB250247)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180683)

Blocking and diluting buffer : 5% NFDM/TBST. Exposure time lanes 1-3 : 7 seconds, lanes 4-6 : 40 seconds.

Lanes 1 - 3:

Western blot - Anti-RAC3 antibody [EPR6680] (<a href='/en-us/products/primary-antibodies/rac3-antibody-epr6680-ab129062'>ab129062</a>) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (<a href='/en-us/products/primary-antibodies/rac1-rac2-rac3-antibody-epr18631-ab180683'>ab180683</a>) at 1/1000 dilution

Lanes 1 and 4:

C-Myc/DDK tagged Human full length RAC1 recombinant protein at 10 ng

Lanes 2 and 5:

His-tagged Human full length RAC2 recombinant protein at 10 ng

Lanes 3 and 6:

C-Myc/DDK tagged Human full length RAC3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18631

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250247 is the carrier-free version of ab180683.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rac1 Rac2 and Rac3 are small GTPases belonging to the Rho family of proteins often referred to as Rac GTPases. These proteins are involved mechanically in the regulation of cell growth cytoskeletal reorganization and the generation of reactive oxygen species. Rac1 has a molecular mass of approximately 21 kDa and Rac proteins share a highly conserved amino acid sequence. Rac1 is ubiquitously expressed while Rac2 is mostly found in hematopoietic cells and Rac3 is expressed in the brain and other tissues.
Biological function summary

Rac proteins play key roles in cell movement adhesion and proliferation. Rac1 Rac2 and Rac3 participate in the formation of lamellipodia which are cellular protrusions important for migration. These proteins are part of complexes involved in actin cytoskeleton dynamics affecting how cells interact with their environment. Different Rac isoforms contribute to the precise control and timing of cellular responses to external stimuli influencing processes such as immune cell function and neuronal development.

Pathways

The Rac proteins integrate into the signaling processes that control actin organization and cell cycle progression. The Rac GTPases interact with pathways such as the PI3K/Akt and MAPK pathways. In these pathways they partner with proteins like PAK (p21-activated kinase) to facilitate changes in the cytoskeleton and promote survival signals. These interactions allow cells to modify their shape and migrate in response to different stimuli enabling a connection between cell signaling and movement.

Rac proteins link to several pathological conditions including cancer and immune-related disorders. Rac1 is often implicated in cancer where its activity affects tumor growth and metastasis through alterations in the cell cycle and motility. Rac2 mutations can lead to immune system dysfunctions impacting immune cell migration and response contributing to conditions like chronic granulomatous disease. The dysregulation of Rac activity often involves interactions with other protein partners like Nox enzymes which are responsible for reactive oxygen species production playing a part in disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles (PubMed : 1643658, PubMed : 22843693, PubMed : 23512198, PubMed : 28886345). Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity (PubMed : 9121475). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed : 1643658). In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity (By similarity). In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX (PubMed : 12695502). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization (By similarity). Required for DSG3 translocation to cell-cell junctions, DSG3-mediated organization of cortical F-actin bundles and anchoring of actin at cell junctions; via interaction with DSG3 (PubMed : 22796473).. Isoform B. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins (PubMed : 14625275). It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction (PubMed : 14625275).
See full target information RAC1

Additional targets

RAC2,RAC3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB187643

Anti-CDC42 antibody [EPR15620]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (AB187643)

  • 4
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1
Primary Antibodies

AB40794

Anti-FAK antibody [EP695Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

  • 5
  • 9

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com