Anti-Rac1b antibody [EPR28989-13] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Rac1b antibody [EPR28989-13] - BSA and Azide free (AB324770)

This data was developed using ab322141, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HCT 116 (PMID : 17363564) PANC-1 (PMID : 31817229) A549 (PMID : 31811797).

The identity of the higher MW bands at approximately 37 kDa & 75 kDa (Lane 2 & Lane 5 respectively) are unknown.

The samples are unboiled.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Rac1b antibody [EPR28989-13] (<a href='/en-us/products/primary-antibodies/rac1b-antibody-epr28989-13-ab322141'>ab322141</a>) at 1/1000 dilution

Lane 1:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Rac1b antibody [EPR28989-13] - BSA and Azide free (AB324770)

This data was developed using ab322141, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Rac1a.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Rac1b antibody [EPR28989-13] (<a href='/en-us/products/primary-antibodies/rac1b-antibody-epr28989-13-ab322141'>ab322141</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human Rac1b expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human Rac1a expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 70s