Rabbit Polyclonal RAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAC2 aa 1 to C-terminus.
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Publications
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- Journal of clinical immunology 42:1280-12922022Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhang,Zhi Chen,Wenyan Li,Qiao Liu,Yanping Wang,Xuemei Chen,Zhirui Tian,Qiuyun Yang,Yunfei An,Zhiyong Zhang,Huawei Mao,Xuemei Tang,Ge Lv,Xiaodong ZhaoPubMed 35596857
- Haematologica 106:404-4112021A gain-of-function RAC2 mutation is associated with bone-marrow hypoplasia and an autosomal dominant form of severe combined immunodeficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChantal Lagresle-Peyrou,Aurélien Olichon,Hanem Sadek,Philippe Roche,Claudine Tardy,Cindy Da Silva,Alexandrine Garrigue,Alain Fischer,Despina Moshous,Yves Collette,Capucine Picard,Jean Laurent Casanova,Isabelle André,Marina CavazzanaPubMed 31919089
- Medical science monitor : international medical jo 26:e9191502020Effect of Grilled Nux Vomica on Differential RNA Expression Profile of Gastrocnemius Muscle and Toll‑Like Receptor 4 (TLR-4)/Nuclear Factor kappa B (NF-κB) Signaling in Experimental Autoimmune Myasthenia Gravis Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Hong Jiang et. al.PubMed 32052794
- International journal of biological macromolecules 137:1221-12312019Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Xia et. al.PubMed 31279058
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAC2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15153
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Xenopus tropicalis, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Xenopus tropicalis, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Alternative names
Ras-related C3 botulinum toxin substrate 2, GX, Small G protein, p21-Rac2, RAC2
Recommended products
Rabbit Polyclonal RAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAC2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAC2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15153
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAC2 also known as Ras-related C3 botulinum toxin substrate 2 is a small GTPase with a molecular mass of approximately 21 kDa. RAC2 belongs to the Rho family and facilitates the exchange between active GTP-bound and inactive GDP-bound states influencing various cellular processes. This protein mainly expresses in hematopoietic cells playing essential roles in immune responses. RAC2 transmits signals that regulate cytoskeleton organization cell growth and production of reactive oxygen species particularly in cells of the immune system.
- Biological function summary
RAC2 plays a role in modulating actin cytoskeleton dynamics. RAC2 functions as a part of the NADPH oxidase complex which is pivotal in producing reactive oxygen species in neutrophils. It also impacts cell motility and adhesion processes critical for immune system functionality. By being involved in these cellular processes RAC2 assists neutrophils and macrophages to effectively respond to pathogens. Its expression and function are essential for proper signaling especially in immune cells where rapid responses are necessary.
- Pathways
RAC2 integrates into critical signaling pathways such as the MAPK and PI3K pathways. It interacts with proteins like PAK1 which is involved in cytoskeletal regulation suggesting RAC2's importance in signal transduction relating to cellular movement and immune response. Additionally RAC2 regulates actin polymerization and cellular migration translating external signals into cellular responses. This regulation is important for maintaining proper cellular function and adaptation to environmental changes.
- Associated diseases and disorders
RAC2 has a connection to chronic granulomatous disease (CGD) which results from an impairment in the NADPH oxidase complex leading to reduced ability to produce superoxide radicals. CGD manifests in recurrent infections due to this compromised immune function. Moreover RAC2's involvement in actin dynamics links it to various immunodeficiency disorders. Abnormal RAC2 activity might also connect to other hematological conditions as it influences signaling pathways critical for immune cell proliferation and differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RAC2 antibody (ab154711)
12% SDS PAGE
All lanes: Western blot - Anti-RAC2 antibody (ab154711) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab154711)
Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling RAC2 with ab154711 at 1/500 dilution.
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