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AB191527

Anti-RAC2 antibody

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(3 Publications)

Rabbit Polyclonal RAC2 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RAC2.

View Alternative Names

Ras-related C3 botulinum toxin substrate 2, GX, Small G protein, p21-Rac2, RAC2

1 Images
Western blot - Anti-RAC2 antibody (AB191527)
  • WB

Supplier Data

Western blot - Anti-RAC2 antibody (AB191527)

All lanes:

Western blot - Anti-RAC2 antibody (ab191527)

Lane 1:

Jurkat whole cell lysate

Lane 2:

Sp2/0 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RAC2. The exact immunogen used to generate this antibody is proprietary information.

P15153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab191527 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAC2 also known as Ras-related C3 botulinum toxin substrate 2 is a small GTPase with a molecular mass of approximately 21 kDa. RAC2 belongs to the Rho family and facilitates the exchange between active GTP-bound and inactive GDP-bound states influencing various cellular processes. This protein mainly expresses in hematopoietic cells playing essential roles in immune responses. RAC2 transmits signals that regulate cytoskeleton organization cell growth and production of reactive oxygen species particularly in cells of the immune system.
Biological function summary

RAC2 plays a role in modulating actin cytoskeleton dynamics. RAC2 functions as a part of the NADPH oxidase complex which is pivotal in producing reactive oxygen species in neutrophils. It also impacts cell motility and adhesion processes critical for immune system functionality. By being involved in these cellular processes RAC2 assists neutrophils and macrophages to effectively respond to pathogens. Its expression and function are essential for proper signaling especially in immune cells where rapid responses are necessary.

Pathways

RAC2 integrates into critical signaling pathways such as the MAPK and PI3K pathways. It interacts with proteins like PAK1 which is involved in cytoskeletal regulation suggesting RAC2's importance in signal transduction relating to cellular movement and immune response. Additionally RAC2 regulates actin polymerization and cellular migration translating external signals into cellular responses. This regulation is important for maintaining proper cellular function and adaptation to environmental changes.

RAC2 has a connection to chronic granulomatous disease (CGD) which results from an impairment in the NADPH oxidase complex leading to reduced ability to produce superoxide radicals. CGD manifests in recurrent infections due to this compromised immune function. Moreover RAC2's involvement in actin dynamics links it to various immunodeficiency disorders. Abnormal RAC2 activity might also connect to other hematological conditions as it influences signaling pathways critical for immune cell proliferation and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
See full target information RAC2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1606973 PubMed40881324

2025

ACTN2, regulated by PRDM9, affects the growth and inflammation of vascular smooth muscle cells by interacting with PDLIM1 in intracranial aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxu Zhang,Jinbing Zhao,Zhiqiang Yu,Hongyi Liu

Nature communications 15:2989 PubMed38582902

2024

Gadd45g insufficiency drives the pathogenesis of myeloproliferative neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Peiwen Zhang,Na You,Yiyi Ding,Wenqi Zhu,Nan Wang,Yueqiao Xie,Wanling Huang,Qian Ren,Tiejun Qin,Rongfeng Fu,Lei Zhang,Zhijian Xiao,Tao Cheng,Xiaotong Ma

Molecular cancer therapeutics 23:35-46 PubMed37735104

2023

Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Renata Sano,Mutiah Apatira,Felix DeAnda,Tarikere Gururaja,Muhua Yang,Greta Lundgaard,Chin Pan,Jing Liu,Yongjiao Zhai,Woo Hyun Yoon,Longcheng Wang,Chris Tse,Andrew J Souers,Chih-Hung Lee
View all publications
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