Anti-RAC3 antibody [EPR6680]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (AB129062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1 : 100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (AB129062)

ab129062 (unpurified) at 1/50 dilution staining RAC3 in paraffin-embedded Human human breast carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (AB129062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1 : 100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (AB129062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1 : 100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Lab

Western blot - Anti-RAC3 antibody [EPR6680] (AB129062)

All lanes:

Western blot - Anti-RAC3 antibody [EPR6680] (ab129062) at 1/2000 dilution

Lane 1:

293 cell extract at 10 µg

Lane 2:

293 cell extract at 10 µg with Rac1 peptide

Lane 3:

293 cell extract at 10 µg with Rac2 peptide

Lane 4:

293 cell extract at 10 µg with Rac3 peptide

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 21 kDa

false

• WB

Lab

Western blot - Anti-RAC3 antibody [EPR6680] (AB129062)

All lanes:

Western blot - Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution

All lanes:

Human brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Lab

Western blot - Anti-RAC3 antibody [EPR6680] (AB129062)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution

Lane 1:

Mouse brain lysates at 15 µg

Lane 2:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Lab

Western blot - Anti-RAC3 antibody [EPR6680] (AB129062)

Blocking and diluting buffer : 5% NFDM/TBST. Exposure time lanes 1-3 : 7 seconds, lanes 4-6 : 40 seconds.

Lanes 1 - 3:

Western blot - Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (<a href='/en-us/products/primary-antibodies/rac1-rac2-rac3-antibody-epr18631-ab180683'>ab180683</a>) at 1/1000 dilution

Lanes 1 and 4:

C-Myc/DDK tagged Human full length RAC1 recombinant protein at 10 ng

Lanes 2 and 5:

His-tagged Human full length RAC2 recombinant protein at 10 ng

Lanes 3 and 6:

C-Myc/DDK tagged Human full length RAC3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,25 kDa

false

• WB

Unknown

Western blot - Anti-RAC3 antibody [EPR6680] (AB129062)

All lanes:

Western blot - Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Fetal brain lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

JAR cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RAC3 antibody [EPR6680] (AB129062)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.