Rabbit Recombinant Monoclonal RAC3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129857.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71884.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91674.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272)")
![Western blot - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--western-blot-img439319.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (AB248272)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Predicted | Expected |
| Mouse | Predicted | Expected |
| Rat | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.
Alternative names
Ras-related C3 botulinum toxin substrate 3, p21-Rac3, RAC3
Recommended products
Rabbit Recombinant Monoclonal RAC3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6680
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.2 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248272 is the carrier-free version of Anti-RAC3 antibody [EPR6680] ab129062.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein RAC3 also known as p21-activated kinase 3 or PAK3 is a member of the Rho family of small GTPases. RAC3 has an approximate mass of 22 kDa. This protein functions as a molecular switch that cycles between an active GTP-bound state and an inactive GDP-bound state regulating a variety of cellular processes. RAC3 is expressed in many tissues with prominent expression in the brain muscle and heart. It plays a significant role in controlling the actin cytoskeleton influencing cell shape motility and growth.
- Biological function summary
RAC3 influences cell signaling pathways by interacting with other proteins and forming complexes. It participates in the regulation of a variety of physiological processes including cell polarity migration and growth. RAC3 interacts with proteins such as PAK which is important for the phosphorylation of substrates involved in actin reorganization. This activity allows RAC3 to coordinate with other cellular components affecting processes like the migration of neurons during development and immune cell responses.
- Pathways
RAC3 is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways play important roles in cell proliferation survival and differentiation. Through the MAPK/ERK pathway RAC3 interacts with proteins like Ras and Raf facilitating the signaling cascade that leads to gene expression changes. In the PI3K/AKT pathway RAC3 contributes to cellular survival and metabolism by interacting with PI3K and AKT promoting pathway signaling important for maintaining cell health under stress.
- Associated diseases and disorders
RAC3 has been linked to cancer and neurodevelopmental disorders. Its role in actin cytoskeleton dynamics and cell signaling makes it relevant in the progression of cancers where its overexpression can enhance metastatic capabilities. RAC3 has connections with the epidermal growth factor receptor (EGFR) which enhances proliferative signals in tumor cells. In neurodevelopmental disorders alterations in RAC3 function can impact neuronal migration and brain development. This connection involves RAC3's interplay with proteins such as PAK3 influencing synaptic development and cognitive functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129857.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RAC3 with Purified Anti-RAC3 antibody [EPR6680] ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAC3 antibody [EPR6680] ab129062)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71884.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RAC3 with Purified Anti-RAC3 antibody [EPR6680] ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAC3 antibody [EPR6680] ab129062)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91674.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling RAC3 with Purified Anti-RAC3 antibody [EPR6680] ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAC3 antibody [EPR6680] ab129062)![Western blot - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272), expandable thumbnail](https://content.abcam.com/products/images/rac3-antibody-epr6680-bsa-and-azide-free-ab248272--western-blot-img439319.jpg "Western blot - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)")
Western blot - Anti-RAC3 antibody [EPR6680] - BSA and Azide free (ab248272)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RAC3 antibody [EPR6680] ab129062)
Blocking and diluting buffer: 5% NFDM/TBST. Exposure time lanes 1-3: 7 seconds, lanes 4-6: 40 seconds.
Lanes 1 - 3: Western blot - Anti-RAC3 antibody [EPR6680] (Anti-RAC3 antibody [EPR6680] ab129062) at 1/1000 dilution
Lanes 4 - 6: Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] ab180683) at 1/1000 dilution
Lanes 1 and 4: C-Myc/DDK tagged Human full length RAC1 recombinant protein at 10 ng
Lanes 2 and 5: His-tagged Human full length RAC2 recombinant protein at 10 ng
Lanes 3 and 6: C-Myc/DDK tagged Human full length RAC3 recombinant protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 25 kDa
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