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AB180802

Anti-RACGAP1/MGCRACGAP antibody

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(2 Publications)

Rabbit Polyclonal RACGAP1/MGCRACGAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RACGAP1.

View Alternative Names

KIAA1478, MGCRACGAP, RACGAP1, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolog, MgcRacGAP, CYK4, HsCYK-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (AB180802)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (AB180802)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling RACGAP1/MGCRACGAP with ab180802 (red). DAPI used for nuclear staining (blue).

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB180802)
  • WB

Supplier Data

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB180802)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab180802) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

K562 cell lysate at 25 µg

Lane 3:

Jurkat cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 71 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RACGAP1. The exact immunogen used to generate this antibody is proprietary information.

Q9H0H5

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Alternative Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein RACGAP1 also known as MGCRACGAP is an important component involved in the regulation of cytokinesis. This protein with an approximate mass of 63 kDa exhibits expression in various tissues including high levels in proliferative cell types such as those in tumors and testis. It interacts with Rho family GTPases playing a role in modulating the cytoskeleton during cell division. RACGAP1 localizes at the midbody of dividing cells marking its critical mechanical function in successful cytokinesis completion.
Biological function summary

RACGAP1 plays an essential role in cell cycle regulation and mitotic progression. As a part of the centralspindlin complex it partners with kinesin-like protein CHICA to ensure the accurate completion of cytokinesis. This interaction fosters the stabilization and accumulation of microtubules at the spindle midzone which is fundamental for the cleavage furrow's ingression. RACGAP1's effective modulation of actin dynamics through its GAP activity serves a pivotal function in cytokinetic abscission.

Pathways

RACGAP1 integrates into multiple signaling networks sharing responsibilities in regulating the dynamics of the mitotic spindle and the actomyosin contractile ring. Significant pathways include the Rho signaling pathway and the cell cycle checkpoint signaling. Within these pathways RACGAP1 works closely with proteins such as Aurora B kinase to orchestrate orderly cell division and chromosome segregation ensuring genomic stability.

The altered function of RACGAP1 links to several instances of tumorigenesis as well as certain developmental disorders. Overexpression and dysregulation have correlations with aggressive cancer phenotypes particularly in cases of prostate cancer. RACGAP1's oncogenic role becomes apparent through its interaction with proteins like p53 affecting tumor suppressor pathways. Addressing RACGAP1 anomalies through therapeutic intervention therefore presents a potential strategic target in treating related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis (PubMed : 18511905). Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity (PubMed : 10979956). Has a critical role in erythropoiesis (PubMed : 34818416). Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
See full target information RACGAP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Matrix biology : journal of the International Society for Matrix Biology 81:17-33 PubMed30367951

2018

Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Chellini,Valentina Caprara,Francesca Spadaro,Rosanna Sestito,Anna Bagnato,Laura Rosanò

Cancer science 109:84-93 PubMed29095547

2017

Rac GTPase activating protein 1 promotes oncogenic progression of epithelial ovarian cancer.

Applications

IHC-P

Species

Human

Chuanjiang Wang,Wenxia Wang,Yujuan Liu,Min Yong,Yamei Yang,Honggui Zhou
View all publications
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Product promise

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