AB97315

Anti-RACGAP1/MGCRACGAP antibody

Name: Anti-RACGAP1/MGCRACGAP antibody (ab97315) | Abcam
Brand: Abcam Limited
SKU: AB97315

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(2 Publications)

Rabbit Polyclonal RACGAP1/MGCRACGAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RACGAP1 aa 1-200.

View Alternative Names

KIAA1478, MGCRACGAP, RACGAP1, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolog, MgcRacGAP, CYK4, HsCYK-4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Immunohistochemical analysis of paraffin-embedded H1299 (human non-small cell lung carcinoma cell line) xenograft tissue staining RACGAP1 with ab97315 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Confocal immunofluorescence analysis of paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) whole cells labeling RACGAP1 (green) with ab97315 at 1/500 dilution. Alpha-tubulin filaments (red), at 1 : 2000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining RACGAP1 with ab97315 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Unknown

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 71 kDa

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Supplier Data

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 50 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RACGAP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9H0H5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein RACGAP1 also known as MGCRACGAP is an important component involved in the regulation of cytokinesis. This protein with an approximate mass of 63 kDa exhibits expression in various tissues including high levels in proliferative cell types such as those in tumors and testis. It interacts with Rho family GTPases playing a role in modulating the cytoskeleton during cell division. RACGAP1 localizes at the midbody of dividing cells marking its critical mechanical function in successful cytokinesis completion.

Biological function summary

RACGAP1 plays an essential role in cell cycle regulation and mitotic progression. As a part of the centralspindlin complex it partners with kinesin-like protein CHICA to ensure the accurate completion of cytokinesis. This interaction fosters the stabilization and accumulation of microtubules at the spindle midzone which is fundamental for the cleavage furrow's ingression. RACGAP1's effective modulation of actin dynamics through its GAP activity serves a pivotal function in cytokinetic abscission.

Pathways

RACGAP1 integrates into multiple signaling networks sharing responsibilities in regulating the dynamics of the mitotic spindle and the actomyosin contractile ring. Significant pathways include the Rho signaling pathway and the cell cycle checkpoint signaling. Within these pathways RACGAP1 works closely with proteins such as Aurora B kinase to orchestrate orderly cell division and chromosome segregation ensuring genomic stability.

The altered function of RACGAP1 links to several instances of tumorigenesis as well as certain developmental disorders. Overexpression and dysregulation have correlations with aggressive cancer phenotypes particularly in cases of prostate cancer. RACGAP1's oncogenic role becomes apparent through its interaction with proteins like p53 affecting tumor suppressor pathways. Addressing RACGAP1 anomalies through therapeutic intervention therefore presents a potential strategic target in treating related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis (PubMed : 18511905). Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity (PubMed : 10979956). Has a critical role in erythropoiesis (PubMed : 34818416). Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

See full target information RACGAP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40497948

2025

RacGAP1 Plays an Oncogenic Role in Lung Adenocarcinoma by Regulating the Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Mosca,Mariaceleste Pezzullo,Ilenia De Leo,Anna Truda,Giovanna Marchese,Aniello Russo,Nicoletta Potenza

Cancers 15: PubMed36765657

2023

Cell-Type-Specific Signalling Networks Impacted by Prostate Epithelial-Stromal Intercellular Communication.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberley C Clark,Elizabeth V Nguyen,Birunthi Niranjan,Yunjian Wu,Terry C C Lim Kam Sian,Lisa G Horvath,Renea A Taylor,Roger J Daly

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RACGAP1/MGCRACGAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Western blot - Anti-RACGAP1/MGCRACGAP antibody (AB97315)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise