Rabbit Polyclonal RACGAP1/MGCRACGAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RACGAP1 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RACGAP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0H5
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis (PubMed:18511905). Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity (PubMed:10979956). Has a critical role in erythropoiesis (PubMed:34818416). Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
Alternative names
KIAA1478, MGCRACGAP, RACGAP1, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolog, MgcRacGAP, CYK4, HsCYK-4
Recommended products
Rabbit Polyclonal RACGAP1/MGCRACGAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RACGAP1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RACGAP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0H5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein RACGAP1 also known as MGCRACGAP is an important component involved in the regulation of cytokinesis. This protein with an approximate mass of 63 kDa exhibits expression in various tissues including high levels in proliferative cell types such as those in tumors and testis. It interacts with Rho family GTPases playing a role in modulating the cytoskeleton during cell division. RACGAP1 localizes at the midbody of dividing cells marking its critical mechanical function in successful cytokinesis completion.
- Biological function summary
RACGAP1 plays an essential role in cell cycle regulation and mitotic progression. As a part of the centralspindlin complex it partners with kinesin-like protein CHICA to ensure the accurate completion of cytokinesis. This interaction fosters the stabilization and accumulation of microtubules at the spindle midzone which is fundamental for the cleavage furrow's ingression. RACGAP1's effective modulation of actin dynamics through its GAP activity serves a pivotal function in cytokinetic abscission.
- Pathways
RACGAP1 integrates into multiple signaling networks sharing responsibilities in regulating the dynamics of the mitotic spindle and the actomyosin contractile ring. Significant pathways include the Rho signaling pathway and the cell cycle checkpoint signaling. Within these pathways RACGAP1 works closely with proteins such as Aurora B kinase to orchestrate orderly cell division and chromosome segregation ensuring genomic stability.
- Associated diseases and disorders
The altered function of RACGAP1 links to several instances of tumorigenesis as well as certain developmental disorders. Overexpression and dysregulation have correlations with aggressive cancer phenotypes particularly in cases of prostate cancer. RACGAP1's oncogenic role becomes apparent through its interaction with proteins like p53 affecting tumor suppressor pathways. Addressing RACGAP1 anomalies through therapeutic intervention therefore presents a potential strategic target in treating related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining RACGAP1 with ab97315 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
7.5% SDS-PAGE
All lanes: Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 71 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
Confocal immunofluorescence analysis of paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) whole cells labeling RACGAP1 (green) with ab97315 at 1/500 dilution. Alpha-tubulin filaments (red), at 1:2000 dilution.
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
7.5% SDS PAGE
All lanes: Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution
All lanes: Mouse heart tissue lysate at 50 µg
Predicted band size: 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
Immunohistochemical analysis of paraffin-embedded H1299 (human non-small cell lung carcinoma cell line) xenograft tissue staining RACGAP1 with ab97315 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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