Rabbit Polyclonal RACGAP1/MGCRACGAP phospho S387 antibody. Suitable for WB and reacts with African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human RACGAP1 phospho S387 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human RACGAP1 phospho S387 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0H5
Reactivity data
Select an application| WB | |
|---|---|
| African green monkey | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/500 - 1/1000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis (PubMed:18511905). Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity (PubMed:10979956). Has a critical role in erythropoiesis (PubMed:34818416). Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
Alternative names
KIAA1478, MGCRACGAP, RACGAP1, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolog, MgcRacGAP, CYK4, HsCYK-4
Recommended products
Rabbit Polyclonal RACGAP1/MGCRACGAP phospho S387 antibody. Suitable for WB and reacts with African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human RACGAP1 phospho S387 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human RACGAP1 phospho S387 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0H5
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab193220 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein RACGAP1 also known as MGCRACGAP is an important component involved in the regulation of cytokinesis. This protein with an approximate mass of 63 kDa exhibits expression in various tissues including high levels in proliferative cell types such as those in tumors and testis. It interacts with Rho family GTPases playing a role in modulating the cytoskeleton during cell division. RACGAP1 localizes at the midbody of dividing cells marking its critical mechanical function in successful cytokinesis completion.
- Biological function summary
RACGAP1 plays an essential role in cell cycle regulation and mitotic progression. As a part of the centralspindlin complex it partners with kinesin-like protein CHICA to ensure the accurate completion of cytokinesis. This interaction fosters the stabilization and accumulation of microtubules at the spindle midzone which is fundamental for the cleavage furrow's ingression. RACGAP1's effective modulation of actin dynamics through its GAP activity serves a pivotal function in cytokinetic abscission.
- Pathways
RACGAP1 integrates into multiple signaling networks sharing responsibilities in regulating the dynamics of the mitotic spindle and the actomyosin contractile ring. Significant pathways include the Rho signaling pathway and the cell cycle checkpoint signaling. Within these pathways RACGAP1 works closely with proteins such as Aurora B kinase to orchestrate orderly cell division and chromosome segregation ensuring genomic stability.
- Associated diseases and disorders
The altered function of RACGAP1 links to several instances of tumorigenesis as well as certain developmental disorders. Overexpression and dysregulation have correlations with aggressive cancer phenotypes particularly in cases of prostate cancer. RACGAP1's oncogenic role becomes apparent through its interaction with proteins like p53 affecting tumor suppressor pathways. Addressing RACGAP1 anomalies through therapeutic intervention therefore presents a potential strategic target in treating related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-RACGAP1/MGCRACGAP (phospho S387) antibody (ab193220)
All lanes: Western blot - Anti-RACGAP1/MGCRACGAP (phospho S387) antibody (ab193220) at 1/500 dilution
Lane 1: COS7 tissue extracts
Lane 2: COS7 tissue extracts with antigen-specific peptide
Predicted band size: 71 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com