AB62735

Anti-RACK1 antibody

Name: Anti-RACK1 antibody (ab62735) | Abcam
Brand: Abcam Limited
SKU: AB62735
Price: 435 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(14 Publications)

Rabbit Polyclonal RACK1 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human RACK1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GNB2L1, HLC7, PIG21, RACK1, Small ribosomal subunit protein RACK1, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-like protein 12.3, Human lung cancer oncogene 7 protein, Receptor for activated C kinase, Receptor of activated protein C kinase 1, HLC-7

1 Images

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Western blot - Anti-RACK1 antibody (AB62735)

All lanes:

Western blot - Anti-RACK1 antibody (ab62735) at 1/500 dilution

Lane 1:

Extract from hek-293T cell line

Lane 2:

Extract from HeLa cell line

Lane 3:

Extract from MCF7 cell line

Lane 4:

Extract from U2OS cell line

Lane 5:

Extract from Jurkat cell line

Lane 6:

Extract from RAT1 cell line

Lane 7:

Extract from PC12 cell line

Lane 8:

Extract from NIH3T3 cell line

Secondary

All lanes:

Goat Anti-Rabbit IgG

Predicted band size: 35 kDa

Observed band size: 30 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human RACK1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P63244

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5-10 ÂμL.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5-10 ÂμL.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5-10 ÂμL.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

The antiserum was fractionated and further purified by ion-exchangechromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RACK1 or Receptor for Activated C Kinase 1 is an approximately 36 kDa protein that acts as scaffolding facilitating protein-protein interactions. It has a conserved WD40 repeat domain structure essential for its functions. RACK1 is ubiquitously expressed in various tissues including brain liver and lungs. It aids in assembling signaling complexes by anchoring signaling proteins contributing to various cellular responses.

Biological function summary

The function of RACK1 influences numerous cellular processes such as cell growth migration and apoptosis. It forms part of the ribosome serving a function in translation regulation. RACK1 participates in G-protein mediated signaling as well and binds to activated PKC (protein kinase C) isoforms promoting effective signaling pathways that affect cell behavior.

Pathways

Studies identify RACK1 within the MAPK and JAK/STAT pathways. In the MAPK pathway RACK1 works alongside proteins like ERK1/2 to mediate cellular responses to external stimuli. Within the JAK/STAT pathway its interaction with kinases influences the transcriptional activation of genes involved in immune responses and cell growth signaling emphasizing its role in regulating important cellular activities.

Researchers have linked RACK1 to certain cancers and neurodegenerative diseases. In cancer RACK1's interaction with PKC and other signaling proteins like Src can affect cell proliferation and angiogenesis features significant in tumor progression. Regarding neurodegenerative diseases RACK1's involvement in amyloid precursor protein processing connects it to Alzheimer's disease progression with potential interactions with proteins like presenilin highlighting its role in pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed : 23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed : 28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed : 19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed : 20499158). Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1 (By similarity).. (Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells.. (Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs.. (Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs.. (Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses.

See full target information RACK1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht, Netherlands) 48:1395-1411 PubMed40890537

2025

RACK1 attenuates pancreatic tumorigenesis by suppressing acinar-to-ductal metaplasia through inflammatory signaling modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Tingting Jiang,Huiqing Zhang,Fang Wei,Xiaojia Li,Keping Xie

Molecular cell 81:2808-2822.e10 PubMed34111399

2021

Translation stress and collided ribosomes are co-activators of cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wan,Szymon Juszkiewicz,Daniel Blears,Prashanth Kumar Bajpe,Zhong Han,Peter Faull,Richard Mitter,Aengus Stewart,Ambrosius P Snijders,Ramanujan S Hegde,Jesper Q Svejstrup

Molecular cell 81:1337-1354.e8 PubMed33545068

2021

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Zellner,Martina Schifferer,Christian Behrends

Journal of leukocyte biology 108:1815-1828 PubMed32531836

2020

Tensile force transmitted through LFA-1 bonds mechanoregulate neutrophil inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Vasilios A Morikis,Eman Masadeh,Scott I Simon

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 17:651-663 PubMed31912469

2020

Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2.

Applications

Unspecified application

Species

Unspecified reactive species

Hongzhan Li,Jianqing Shang,Chengliang Zhang,Rulan Lu,Junpao Chen,Xianju Zhou

PLoS pathogens 15:e1008021 PubMed31525236

2019

RACK1 mediates rewiring of intracellular networks induced by hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Seung Lee,Keisuke Tabata,Woan-Ing Twu,Md Shafiqur Rahman,Hee Sun Kim,Jin Bae Yu,Min Hyeok Jee,Ralf Bartenschlager,Sung Key Jang

Cellular signalling 63:109384 PubMed31394193

2019

O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Guoqing Zhu,Ya Liu,Qi Wu,Xiao Zhang,Zhixuan Bian,Yue Zhang,Qiuhui Pan,Fenyong Sun

OncoTargets and therapy 12:4795-4805 PubMed31417279

2019

Upregulated RACK1 attenuates gastric cancer cell growth and epithelial-mesenchymal transition via suppressing Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Zhu,Wen Chen,Guoqing Li,Honghui Chen,Wenqiu Liao,Li Zhang,Xiaoli Xiao

International journal of molecular medicine 40:1965-1970 PubMed29039466

2017

RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Feng,Liyi Xie,Ranran Kong,Yali Zhang,Kehui Shi,Wanhong Lu,Hongli Jiang

International journal of molecular medicine 37:251-7 PubMed26718491

2016

RACK1 affects glioma cell growth and differentiation through the CNTN2-mediated RTK/Ras/MAPK pathway.

Applications

Species

Human

Yu Yan,Yugang Jiang

View all publications

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Product promise

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Anti-RACK1 antibody

Western blot - Anti-RACK1 antibody (AB62735)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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