AB3620

Anti-Rad17 (phospho S645) antibody

Name: Anti-Rad17 (phospho S645) antibody (ab3620) | Abcam
Brand: Abcam Limited
SKU: AB3620

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(5 Publications)

Rabbit Polyclonal RAD17 phospho S645 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RAD17 pS645.

View Alternative Names

R24L, RAD17, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (AB3620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (AB3620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Rad17 with ab3620 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (AB3620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling Rad17 with ab3620 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Rad17 (phospho S645) antibody (AB3620)

Lower panel : membrane was reblotted with goat anti-Rad17 antibody to examine total Rad17.

All lanes:

Western blot - Anti-Rad17 (phospho S645) antibody (ab3620) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate treated with Etoposide at 50 µg

Lane 2:

HeLa whole cell lysate mock-treated at 50 µg

Lane 3:

HEK293T whole cell lysate treated with Etoposide at 50 µg

Lane 4:

HEK293T whole cell lysate mock-treated at 50 µg

Predicted band size: 77 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RAD17 pS645. The exact immunogen used to generate this antibody is proprietary information.

O75943

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for Rad 17pSer 645 were affinity purified using the phospho peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad17 also known as CCYC1 is a protein involved in DNA damage checkpoint control. It has an approximate molecular weight of 75 kDa. Rad17 is expressed in various tissues with higher levels in proliferating cells such as cancerous tissues. It functions as a part of the replication factor C (RFC) complex delivering its essential role during the DNA replication and damage response.

Biological function summary

Rad17 plays an important role in maintaining genome stability by activating cell cycle checkpoints. It belongs to the RFC-like complex which identifies DNA damage and initiates the DNA repair process. The complex binds to chromatin and helps recruit the 9-1-1 complex (Rad9-Rad1-Hus1) to sites of DNA damage. This action is important for the cell to halt the cell cycle and commence repair preventing mutations from being passed on during cell division.

Pathways

Rad17 functions in the DNA damage response pathway and is involved in the ATR-Chk1 pathway. Rad17 recruits ATR (ataxia-telangiectasia and Rad3-related protein) to stalled replication forks facilitating the phosphorylation and activation of Chk1 a checkpoint kinase. This signal allows cells to pause the replication process and repair DNA before continuing the cell cycle. Rad17 is closely related to proteins such as ATR and Chk1 through these pathways as they work together to maintain cellular integrity.

Rad17 has links to cancer and genomic instability syndromes. Mutations or malfunctions in Rad17 can disrupt normal checkpoint control and repair mechanisms leading to uncontrolled cell proliferation and tumor development. In cancer Rad17 interacts with proteins such as BRCA1 in pathways that emphasize DNA repair and checkpoint activation. BRCA1 and Rad17 coordinate to preserve genomic stability and disruptions in these proteins' functions can contribute to the onset of certain breast and ovarian cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Has a weak ATPase activity required for binding to chromatin (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (PubMed : 21659603). Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites (PubMed : 24534091). May also serve as a sensor of DNA replication progression (PubMed : 12578958, PubMed : 14500819, PubMed : 15538388).

See full target information RAD17 pS645

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy :1-9 PubMed32660328

2020

DGUOK-AS1 promotes the proliferation cervical cancer through regulating miR-653-5p/EMSY.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Yan,Guanhao Chen,Jichan Nie

Nucleic acids research 47:4068-4085 PubMed30799487

2019

Depletion of the RNA binding protein HNRNPD impairs homologous recombination by inhibiting DNA-end resection and inducing R-loop accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Luigi Alfano,Antonella Caporaso,Angela Altieri,Milena Dell'Aquila,Claudia Landi,Luca Bini,Francesca Pentimalli,Antonio Giordano

Cell cycle (Georgetown, Tex.) 11:1796-803 PubMed22453082

2012

ATM-dependent phosphorylation of the checkpoint clamp regulates repair pathways and maintains genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Min Hwa Shin,Ming Yuan,Hao Zhang,Joseph B Margolick,Mihoko Kai

Proceedings of the National Academy of Sciences of 107:7491-6 PubMed20368419

2010

Nuclear phosphoinositide 3-kinase beta controls double-strand break DNA repair.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Amit Kumar,Oscar Fernandez-Capetillo,Oscar Fernadez-Capetillo,Ana C Carrera

Journal of virology 79:569-80 PubMed15596849

2004

Viral transport of DNA damage that mimics a stalled replication fork.

Applications

Unspecified application

Species

Unspecified reactive species

Jaana Jurvansuu,Kenneth Raj,Andrzej Stasiak,Peter Beard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Rad17 (phospho S645) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (AB3620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (AB3620)

Western blot - Anti-Rad17 (phospho S645) antibody (AB3620)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise