AB284716

Anti-Rad17 (phospho S645) antibody [EP1519Y] - BSA and Azide free

RabMAb
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Rabbit Monoclonal RAD17 phospho S645 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

R24L, RAD17, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

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Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y] - BSA and Azide free (AB284716)

This data was developed using ab75779, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y] - BSA and Azide free (ab284716) at 1/5000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Unconjugated

Anti-Rad17 (phospho S645) antibody [EP1519Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1519Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab284716 is the carrier-free version of ab75779

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad17 also known as CCYC1 is a protein involved in DNA damage checkpoint control. It has an approximate molecular weight of 75 kDa. Rad17 is expressed in various tissues with higher levels in proliferating cells such as cancerous tissues. It functions as a part of the replication factor C (RFC) complex delivering its essential role during the DNA replication and damage response.

Biological function summary

Rad17 plays an important role in maintaining genome stability by activating cell cycle checkpoints. It belongs to the RFC-like complex which identifies DNA damage and initiates the DNA repair process. The complex binds to chromatin and helps recruit the 9-1-1 complex (Rad9-Rad1-Hus1) to sites of DNA damage. This action is important for the cell to halt the cell cycle and commence repair preventing mutations from being passed on during cell division.

Pathways

Rad17 functions in the DNA damage response pathway and is involved in the ATR-Chk1 pathway. Rad17 recruits ATR (ataxia-telangiectasia and Rad3-related protein) to stalled replication forks facilitating the phosphorylation and activation of Chk1 a checkpoint kinase. This signal allows cells to pause the replication process and repair DNA before continuing the cell cycle. Rad17 is closely related to proteins such as ATR and Chk1 through these pathways as they work together to maintain cellular integrity.

Rad17 has links to cancer and genomic instability syndromes. Mutations or malfunctions in Rad17 can disrupt normal checkpoint control and repair mechanisms leading to uncontrolled cell proliferation and tumor development. In cancer Rad17 interacts with proteins such as BRCA1 in pathways that emphasize DNA repair and checkpoint activation. BRCA1 and Rad17 coordinate to preserve genomic stability and disruptions in these proteins' functions can contribute to the onset of certain breast and ovarian cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Has a weak ATPase activity required for binding to chromatin (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (PubMed : 21659603). Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites (PubMed : 24534091). May also serve as a sensor of DNA replication progression (PubMed : 12578958, PubMed : 14500819, PubMed : 15538388).

See full target information RAD17 phospho S645

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rad17 (phospho S645) antibody [EP1519Y] - BSA and Azide free

Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y] - BSA and Azide free (AB284716)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise