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AB247429

Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAD17 phospho S656 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

R24L, RAD17, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)

This data was developed using ab76012, the same antibody clone in a different buffer formulation.

ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)

This data was developed using ab76012, the same antibody clone in a different buffer formulation.ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.

Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)
  • WB

Unknown

Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (AB247429)

This data was developed using ab76012, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (<a href='/en-us/products/primary-antibodies/rad17-phospho-s656-antibody-epr3145-ab76012'>ab76012</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3145

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad17 also known as CCYC1 is a protein involved in DNA damage checkpoint control. It has an approximate molecular weight of 75 kDa. Rad17 is expressed in various tissues with higher levels in proliferating cells such as cancerous tissues. It functions as a part of the replication factor C (RFC) complex delivering its essential role during the DNA replication and damage response.
Biological function summary

Rad17 plays an important role in maintaining genome stability by activating cell cycle checkpoints. It belongs to the RFC-like complex which identifies DNA damage and initiates the DNA repair process. The complex binds to chromatin and helps recruit the 9-1-1 complex (Rad9-Rad1-Hus1) to sites of DNA damage. This action is important for the cell to halt the cell cycle and commence repair preventing mutations from being passed on during cell division.

Pathways

Rad17 functions in the DNA damage response pathway and is involved in the ATR-Chk1 pathway. Rad17 recruits ATR (ataxia-telangiectasia and Rad3-related protein) to stalled replication forks facilitating the phosphorylation and activation of Chk1 a checkpoint kinase. This signal allows cells to pause the replication process and repair DNA before continuing the cell cycle. Rad17 is closely related to proteins such as ATR and Chk1 through these pathways as they work together to maintain cellular integrity.

Rad17 has links to cancer and genomic instability syndromes. Mutations or malfunctions in Rad17 can disrupt normal checkpoint control and repair mechanisms leading to uncontrolled cell proliferation and tumor development. In cancer Rad17 interacts with proteins such as BRCA1 in pathways that emphasize DNA repair and checkpoint activation. BRCA1 and Rad17 coordinate to preserve genomic stability and disruptions in these proteins' functions can contribute to the onset of certain breast and ovarian cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Has a weak ATPase activity required for binding to chromatin (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (PubMed : 21659603). Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites (PubMed : 24534091). May also serve as a sensor of DNA replication progression (PubMed : 12578958, PubMed : 14500819, PubMed : 15538388).
See full target information RAD17 phospho S656
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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