JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326664

Anti-Rad17 (phospho S656) antibody [SR2259]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RAD17 phospho S656 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

R24L, RAD17, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [SR2259] (AB326664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [SR2259] (AB326664)

Formalin-fixed, paraffin-embedded human lung tissue stained for Rad17 (phospho S656) using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Rad17 (phospho S656) antibody [SR2259] (AB326664)
  • WB

Supplier Data

Western blot - Anti-Rad17 (phospho S656) antibody [SR2259] (AB326664)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HeLa cell treated with CA lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 77 kDa
Observed band size : 85 kDa

Exposure time : 13 seconds

All lanes:

Western blot - Anti-Rad17 (phospho S656) antibody [SR2259] (ab326664) at 1/1000 dilution

Lane 1:

HeLa cell treated with CA lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 85 kDa

false

Exposure time: 13s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR2259

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Has a weak ATPase activity required for binding to chromatin (PubMed : 10208430, PubMed : 11418864, PubMed : 11687627, PubMed : 11799063, PubMed : 12672690, PubMed : 14624239, PubMed : 15235112). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (PubMed : 21659603). Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites (PubMed : 24534091). May also serve as a sensor of DNA replication progression (PubMed : 12578958, PubMed : 14500819, PubMed : 15538388).
See full target information RAD17 pS656
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com