AB188235

Anti-RAD18 antibody

Name: Anti-RAD18 antibody (ab188235) | Abcam
Brand: Abcam Limited
SKU: AB188235

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(9 Publications)

Rabbit Polyclonal RAD18 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD18 aa 250 to C-terminus.

View Alternative Names

RNF73, RAD18, E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73, RING-type E3 ubiquitin transferase RAD18, hHR18, hRAD18

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunofluorescent analysis of RAD18 expression in U2OS control cells and RAD18 siRNA treated U2OS cells, using ab188235 at 1/2000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunofluorescent analysis U2OS cells fixed in 3% PFA 10 mins and permeabilised with methanol 2 mins irradiated with 3Gy IR for 1.5h labeling RAD18 with ab188235 (1), DNA (2) and merged (3)

Supplier Data

Immunoprecipitation - Anti-RAD18 antibody (AB188235)

Western blot analysis (using ab188235) of immunoprecipitation from U2OS cell lysates(lysated in 0.5% NP40)

Lane 1 : Input

Lane 2 : immunoprecipitate with control serum+protein A sepharose.

Lane 3 : immunoprecipitate with ab188235+ protein A sepharose.

All lanes:

Immunoprecipitation - Anti-RAD18 antibody (ab188235)

Predicted band size: 56 kDa

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Supplier Data

Western blot - Anti-RAD18 antibody (AB188235)

All lanes:

Western blot - Anti-RAD18 antibody (ab188235) at 1/2000 dilution

Lane 1:

Control U2OS cell lysate at 40 µg

Lane 2:

RAD18 si treated U2OS cell lysate at 40 µg

Lane 3:

NIH-3T3 cell lysate at 40 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human RAD18 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NS91

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "Fixation with 3% PFA 10 min, RT and afterwards permeabilization with methanol for 2 min." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD18 also known as E3 ubiquitin-protein ligase RAD18 weighs approximately 56 kDa. It functions as a E3 ubiquitin ligase and is key to the post-replication repair (PRR) pathway. RAD18 is ubiquitously expressed across many tissues including the testis heart and thymus indicating its fundamental role in DNA repair processes. Its primary mechanical job involves the monoubiquitination of PCNA a process important for DNA damage bypass.

Biological function summary

The protein interacts with other repair proteins to ensure the stability of the genome. RAD18 forms part of the larger PRR pathway complex engaging with partner proteins like RAD6 to facilitate the repair of damaged DNA. It orchestrates the recruitment of translesion synthesis (TLS) polymerases at sites of damage playing an instrumental role in resolving stalled replication forks an important aspect of maintaining cellular health and replication fidelity.

Pathways

A significant role emerges for RAD18 in the DNA damage repair and cell cycle checkpoint pathways. Within these pathways RAD18 interacts closely with members of the RAD6 and FANCD2 proteins. It acts as a bridge connecting ubiquitin-mediated signaling and the activation of downstream proteins required for efficient DNA lesion bypass. This highlights its pivotal position in preserving genomic integrity and cellular division processes.

RAD18's malfunction links to cancer development and Fanconi anemia. Its impaired function in the DNA damage repair cascade can lead to genomic instability a hallmark of cancer progression. Additionally through pathways that involve the FANCD2 protein RAD18 deficiencies have been associated with the symptoms of Fanconi anemia a disorder characterized by bone marrow failure and cancer predisposition. Understanding RAD18's role furthers insights into therapeutic strategies for such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

See full target information RAD18

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:7066 PubMed38528023

2024

Role of Rad18 in B cell activation and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Kalweit,Vanessa Gölling,Christian Kosan,Berit Jungnickel

Cell reports 42:112296 PubMed36961817

2023

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination.

Applications

Species

Human

Yi-Chang Wang,Andrew A Kelso,Adak Karamafrooz,Yi-Hsuan Chen,Wei-Kai Chen,Chun-Ting Cheng,Yue Qi,Long Gu,Linda Malkas,Angelo Taglialatela,Hsing-Jien Kung,George-Lucian Moldovan,Alberto Ciccia,Jeremy M Stark,David K Ann

Nucleic acids research 50:8060-8079 PubMed35849344

2022

LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.

Applications

Species

Human

Ruyuan Yu,Yameng Hu,Shuxia Zhang,Xincheng Li,Miaoling Tang,Meisongzhu Yang,Xingui Wu,Ziwen Li,Xinyi Liao,Yingru Xu,Man Li,Suwen Chen,Wanying Qian,Li-Yun Gong,Libing Song,Jun Li

Journal of biochemistry : PubMed33508099

2021

RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.

Applications

ICC

Species

Human

Md Kawsar Mustofa,Yuki Tanoue,Mami Chirifu,Tatsuya Shimasaki,Chie Tateishi,Teruya Nakamura,Satoshi Tateishi

Archives of gynecology and obstetrics 302:1243-1254 PubMed32875345

2020

Long non-coding RNA LINC00858 aggravates the oncogenic phenotypes of ovarian cancer cells through miR-134-5p/RAD18 signaling.

Applications

Species

Human

Heng Xue,Zhihui Wu,Dongdong Rao,Bimin Zhuo,Qingquan Chen

Oncology reports 44:213-223 PubMed32319669

2020

RAD18 promotes colorectal cancer metastasis by activating the epithelial‑mesenchymal transition pathway.

Applications

WB, IHC

Species

Human, Human

Peng Li,Chao He,Aidi Gao,Xueqi Yan,Xiaochun Xia,Jundong Zhou,Jinchang Wu

Genes & development 33:1702-1717 PubMed31699778

2019

Crosstalk between Lys63- and Lys11-polyubiquitin signaling at DNA damage sites is driven by Cezanne.

Applications

WB, IF

Species

Human, Human

Xiao Wu,Shichang Liu,Cari Sagum,Jianji Chen,Rajesh Singh,Apurva Chaturvedi,John R Horton,Tanuja R Kashyap,David Fushman,Xiaodong Cheng,Mark T Bedford,Bin Wang

Nature cell biology 21:487-497 PubMed30804506

2019

53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage.

Applications

WB, IF

Species

Human, Human

Julian Spies,Claudia Lukas,Kumar Somyajit,Maj-Britt Rask,Jiri Lukas,Kai John Neelsen

The Journal of biological chemistry 290:11853-64 PubMed25787082

2015

Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs.

Applications

IHC

Species

Mouse

Xiangbing Meng,Shujie Yang,Yuping Zhang,Xinjun Wang,Renee X Goodfellow,Yichen Jia,Kristina W Thiel,Henry D Reyes,Baoli Yang,Kimberly K Leslie

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RAD18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

Immunoprecipitation - Anti-RAD18 antibody (AB188235)

Western blot - Anti-RAD18 antibody (AB188235)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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