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AB79763

Anti-RAD18 antibody

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(6 Publications)

Rabbit Polyclonal RAD18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD18.

View Alternative Names

RNF73, RAD18, E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73, RING-type E3 ubiquitin transferase RAD18, hHR18, hRAD18

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB79763)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB79763)

Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.

Western blot - Anti-RAD18 antibody (AB79763)
  • WB

Unknown

Western blot - Anti-RAD18 antibody (AB79763)

The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.

All lanes:

Western blot - Anti-RAD18 antibody (ab79763) at 1/1000 dilution

All lanes:

A549 crude extract

Predicted band size: 56 kDa

Observed band size: 70 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RAD18. The exact immunogen used to generate this antibody is proprietary information.

Q9NS91

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6 - 8.5 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD18 also known as E3 ubiquitin-protein ligase RAD18 weighs approximately 56 kDa. It functions as a E3 ubiquitin ligase and is key to the post-replication repair (PRR) pathway. RAD18 is ubiquitously expressed across many tissues including the testis heart and thymus indicating its fundamental role in DNA repair processes. Its primary mechanical job involves the monoubiquitination of PCNA a process important for DNA damage bypass.
Biological function summary

The protein interacts with other repair proteins to ensure the stability of the genome. RAD18 forms part of the larger PRR pathway complex engaging with partner proteins like RAD6 to facilitate the repair of damaged DNA. It orchestrates the recruitment of translesion synthesis (TLS) polymerases at sites of damage playing an instrumental role in resolving stalled replication forks an important aspect of maintaining cellular health and replication fidelity.

Pathways

A significant role emerges for RAD18 in the DNA damage repair and cell cycle checkpoint pathways. Within these pathways RAD18 interacts closely with members of the RAD6 and FANCD2 proteins. It acts as a bridge connecting ubiquitin-mediated signaling and the activation of downstream proteins required for efficient DNA lesion bypass. This highlights its pivotal position in preserving genomic integrity and cellular division processes.

RAD18's malfunction links to cancer development and Fanconi anemia. Its impaired function in the DNA damage repair cascade can lead to genomic instability a hallmark of cancer progression. Additionally through pathways that involve the FANCD2 protein RAD18 deficiencies have been associated with the symptoms of Fanconi anemia a disorder characterized by bone marrow failure and cancer predisposition. Understanding RAD18's role furthers insights into therapeutic strategies for such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
See full target information RAD18
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:345-351 PubMed36634340

2023

Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mikio Shimada,Takumi Tokumiya,Tomoko Miyake,Kaima Tsukada,Norie Kanzaki,Hiromi Yanagihara,Junya Kobayashi,Yoshihisa Matsumoto

Nature chemical biology 12:22-8 PubMed26595461

2015

CRISPR-Cas9-based target validation for p53-reactivating model compounds.

Applications

WB

Species

Unspecified reactive species

Michael Wanzel,Jonas B Vischedyk,Miriam P Gittler,Niklas Gremke,Julia R Seiz,Mirjam Hefter,Magdalena Noack,Rajkumar Savai,Marco Mernberger,Joël P Charles,Jean Schneikert,Anne Catherine Bretz,Andrea Nist,Thorsten Stiewe

The Journal of cell biology 206:183-97 PubMed25023518

2014

DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis.

Applications

Unspecified application

Species

Human

Michelle K Zeman,Jia-Ren Lin,Raimundo Freire,Karlene A Cimprich

The EMBO journal 33:1698-712 PubMed24966277

2014

Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses.

Applications

WB

Species

Human

Min Peng,Jenny Xie,Anna Ucher,Janet Stavnezer,Sharon B Cantor

The EMBO journal 31:908-18 PubMed22157819

2011

Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability.

Applications

WB

Species

Human

Mathew Jk Jones,Luca Colnaghi,Tony T Huang

The Journal of cell biology 194:177-86 PubMed21768287

2011

APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage.

Applications

WB

Species

Human

Xiomaris M Cotto-Rios,Mathew J K Jones,Luca Busino,Michele Pagano,Tony T Huang
View all publications
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Product promise

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