JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB157463

Anti-RAD18 antibody [EPR8942]

1

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal RAD18 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RNF73, RAD18, E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73, RING-type E3 ubiquitin transferase RAD18, hHR18, hRAD18

2 Images
Flow Cytometry (Intracellular) - Anti-RAD18 antibody [EPR8942] (AB157463)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RAD18 antibody [EPR8942] (AB157463)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling RAD18 with ab157463 at 1/10 dilution (red) compared to a rabbit IgG negative control antibody (green).

Western blot - Anti-RAD18 antibody [EPR8942] (AB157463)
  • WB

Unknown

Western blot - Anti-RAD18 antibody [EPR8942] (AB157463)

All lanes:

Western blot - Anti-RAD18 antibody [EPR8942] (ab157463) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HCT-116 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8942

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB188235

Anti-RAD18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB188235)

  • 0
  • 0
Primary Antibodies

AB79763

Anti-RAD18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (AB79763)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB249303

Anti-UPB1 antibody [EPR9132] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-UPB1 antibody [EPR9132] - BSA and Azide free (AB249303)

  • 0
  • 0
Proteins & Peptides

AB112417

Recombinant Human RAD18 protein

SDS-PAGE - Recombinant Human RAD18 protein (AB112417)

  • 0
  • 0
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD18 also known as E3 ubiquitin-protein ligase RAD18 weighs approximately 56 kDa. It functions as a E3 ubiquitin ligase and is key to the post-replication repair (PRR) pathway. RAD18 is ubiquitously expressed across many tissues including the testis heart and thymus indicating its fundamental role in DNA repair processes. Its primary mechanical job involves the monoubiquitination of PCNA a process important for DNA damage bypass.
Biological function summary

The protein interacts with other repair proteins to ensure the stability of the genome. RAD18 forms part of the larger PRR pathway complex engaging with partner proteins like RAD6 to facilitate the repair of damaged DNA. It orchestrates the recruitment of translesion synthesis (TLS) polymerases at sites of damage playing an instrumental role in resolving stalled replication forks an important aspect of maintaining cellular health and replication fidelity.

Pathways

A significant role emerges for RAD18 in the DNA damage repair and cell cycle checkpoint pathways. Within these pathways RAD18 interacts closely with members of the RAD6 and FANCD2 proteins. It acts as a bridge connecting ubiquitin-mediated signaling and the activation of downstream proteins required for efficient DNA lesion bypass. This highlights its pivotal position in preserving genomic integrity and cellular division processes.

RAD18's malfunction links to cancer development and Fanconi anemia. Its impaired function in the DNA damage repair cascade can lead to genomic instability a hallmark of cancer progression. Additionally through pathways that involve the FANCD2 protein RAD18 deficiencies have been associated with the symptoms of Fanconi anemia a disorder characterized by bone marrow failure and cancer predisposition. Understanding RAD18's role furthers insights into therapeutic strategies for such conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
See full target information RAD18
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:292 PubMed38976080

2024

Nuclear translocation of ISG15 regulated by PPP2R2B inhibits cisplatin resistance of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gaowei Huang,Jinwen Liu,Anze Yu,Chenggong Luo,Jiangquan Zhu,Yinghan Wang,Ziran Dai,Lizhen Zhang,Zihao Feng,Jun Lu,Zhong Dong,Junhang Luo,Wei Chen,Zhenhua Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com