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AB154769

Anti-Rad21 antibody

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(37 Publications)

Anti-Rad21 antibody (ab154769) is a rabbit polyclonal antibody detecting Rad21 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Over 20 publications

View Alternative Names

HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

Immunohistochemical analysis of paraffin-embedded human lung papillary adenocarcinoma tissue stained for RAD21 protein at nucleus in human lung papillary adenocarcinoma with ab154769 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (AB154769)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (AB154769)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Rad21 protein at nucleus using ab154769 at a 1/1000 dilution (Green). phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red).

Immunoprecipitation - Anti-Rad21 antibody (AB154769)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rad21 antibody (AB154769)

Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 µg of ab154769. Western blot analysis was performed using ab154769.

All lanes:

Immunoprecipitation - Anti-Rad21 antibody (ab154769) at 5 µg

Lane 1:

Jurkat whole cell extracts (Input)

Lane 2:

Jurkat whole cell extracts with control IgG (-)

Lane 3:

Jurkat whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 72 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue stained for Rad21 protein at nucleus with ab154769 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Rad21 protein at nucleus with ab154769 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Rad21 antibody (AB154769)
  • WB

Supplier Data

Western blot - Anti-Rad21 antibody (AB154769)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Rad21 antibody (ab154769) at 1/1000 dilution

Lane 1:

K562 whole cell lysates at 30 µg

Lane 2:

THP-1 whole cell lysates at 30 µg

Lane 3:

HL-60 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72 kDa

false

Western blot - Anti-Rad21 antibody (AB154769)
  • WB

Supplier Data

Western blot - Anti-Rad21 antibody (AB154769)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Rad21 antibody (ab154769) at 1/1000 dilution

Lane 1:

C2C12 whole cell lysates at 30 µg

Lane 2:

NIH-3T3 whole cell lysates at 30 µg

Lane 3:

C6 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RAD21 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

O60216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Rad21 antibody (ab154769) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Rad21?
Anti-Rad21 (ab154769) specifically detects a band for Rad21 (UniProt: O60216) at a molecular weight of 72kDa.

Trusted by the scientific community
Anti-Rad21 (ab154769) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
Biological function summary

Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.

Pathways

Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.

Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed : 11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed : 11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed : 25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).. 64-kDa C-terminal product. May promote apoptosis.
See full target information RAD21
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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 33:5 PubMed40183884

2025

TOP2B is required for compartment strength changes upon retinoic acid treatment in SH-SY5Y cells.

Applications

Unspecified application

Species

Unspecified reactive species

Erica M Hildebrand,Ian G Cowell,Mushtaq M Khazeem,Snehal Sambare,Ozgun Uyan,Job Dekker,Caroline A Austin

Scientific reports 15:9918 PubMed40121293

2025

Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Keun P Kim

Science advances 10:eadq0987 PubMed39671497

2024

Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Angeliki Platania,Cathie Erb,Mariano Barbieri,Bastien Molcrette,Erwan Grandgirard,Marit A C de Kort,Wim Pomp,Karen Meaburn,Tiegh Taylor,Virlana M Shchuka,Silvia Kocanova,Mariia Nazarova,Guilherme Monteiro Oliveira,Jennifer A Mitchell,Evi Soutoglou,Tineke L Lenstra,Nacho Molina,Argyris Papantonis,Kerstin Bystricky,Tom Sexton

Nature communications 15:7258 PubMed39179577

2024

Cooperative insulation of regulatory domains by CTCF-dependent physical insulation and promoter competition.

Applications

Unspecified application

Species

Unspecified reactive species

Thais Ealo,Victor Sanchez-Gaya,Patricia Respuela,María Muñoz-San Martín,Elva Martin-Batista,Endika Haro,Alvaro Rada-Iglesias

Nature communications 14:7947 PubMed38040722

2023

Defining a core configuration for human centromeres during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ayantika Sen Gupta,Chris Seidel,Dai Tsuchiya,Sean McKinney,Zulin Yu,Sarah E Smith,Jay R Unruh,Jennifer L Gerton

Nature communications 14:7762 PubMed38040699

2023

SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Qing Liu,Irene Paassen,Lars Custers,Peter Zeller,Hans Teunissen,Dilara Ayyildiz,Jiayou He,Juliane Laura Buhl,Eelco Wieger Hoving,Alexander van Oudenaarden,Elzo de Wit,Jarno Drost

Nature immunology 25:129-141 PubMed37985858

2023

BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin.

Applications

Unspecified application

Species

Unspecified reactive species

Malay Mandal,Mark Maienschein-Cline,Yeguang Hu,Azam Mohsin,Margaret L Veselits,Nathaniel E Wright,Michael K Okoreeh,Young Me Yoon,Jacob Veselits,Katia Georgopoulos,Marcus R Clark

Nucleic acids research 51:9594-9609 PubMed37702151

2023

CRISPR screens in sister chromatid cohesion defective cells reveal PAXIP1-PAGR1 as regulator of chromatin association of cohesin.

Applications

Unspecified application

Species

Unspecified reactive species

Janne J M van Schie,Klaas de Lint,Thom M Molenaar,Macarena Moronta Gines,Jesper A Balk,Martin A Rooimans,Khashayar Roohollahi,Govind M Pai,Lauri Borghuis,Anisha R Ramadhin,Francesco Corazza,Josephine C Dorsman,Kerstin S Wendt,Rob M F Wolthuis,Job de Lange

Molecular cell 83:1377-1392.e6 PubMed37146570

2023

Loop stacking organizes genome folding from TADs to chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Antonina Hafner,Minhee Park,Scott E Berger,Sedona E Murphy,Elphège P Nora,Alistair N Boettiger

Science advances 9:eade3876 PubMed36857449

2023

Cohesin regulates alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Amit K Singh,Qingrong Chen,Cu Nguyen,Daoud Meerzaman,Dinah S Singer
View all publications
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