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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal RAD21 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD21 aa 1-400.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
IHC-P | ICC/IF | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Double-strand-break repair protein rad21 homologAs a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).64-kDa C-terminal productMay promote apoptosis.
Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1, NXP1, KIAA0078, HR21, SCC1, RAD21
Rabbit Polyclonal RAD21 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD21 aa 1-400.
Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1, NXP1, KIAA0078, HR21, SCC1, RAD21
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.
Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.
Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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7.5% SDS PAGE
All lanes: Western blot - Anti-Rad21 antibody (AB154769) at 1/1000 dilution
Lane 1: K562 whole cell lysate at 30 µg
Lane 2: THP1 whole cell lysate at 30 µg
Lane 3: HL60 whole cell lysate at 30 µg
Lane 4: NCI-H929 whole cell lysate at 30 µg
Predicted band size: 72 kDa
Observed band size: 130 kDa
Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
7.5% SDS PAGE
All lanes: Western blot - Anti-Rad21 antibody (AB154769) at 1/3000 dilution
Lane 1: NIH3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Predicted band size: 72 kDa
Observed band size: 130 kDa
Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
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