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Rabbit Polyclonal RAD21 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD21 aa 1-400.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human RAD21 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link O60216

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Target data

Function

Double-strand-break repair protein rad21 homologAs a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).64-kDa C-terminal productMay promote apoptosis.

Alternative names

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Rabbit Polyclonal RAD21 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAD21 aa 1-400.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human RAD21 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link O60216
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.

Activity summary

Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.

Pathways

Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.

Associated diseases and disorders

Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Rad21 antibody (ab154769), expandable thumbnail

    Western blot - Anti-Rad21 antibody (ab154769)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Rad21 antibody (AB154769) at 1/1000 dilution

    Lane 1: K562 whole cell lysate at 30 µg

    Lane 2: THP1 whole cell lysate at 30 µg

    Lane 3: HL60 whole cell lysate at 30 µg

    Lane 4: NCI-H929 whole cell lysate at 30 µg

    Predicted band size: 72 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)

    Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (ab154769), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (ab154769)

    Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.

  • Western blot - Anti-Rad21 antibody (ab154769), expandable thumbnail

    Western blot - Anti-Rad21 antibody (ab154769)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Rad21 antibody (AB154769) at 1/3000 dilution

    Lane 1: NIH3T3 whole cell lysate at 30 µg

    Lane 2: JC whole cell lysate at 30 µg

    Lane 3: BCL-1 whole cell lysate at 30 µg

    Predicted band size: 72 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)

    Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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