Anti-Rad21 antibody (ab154769) is a rabbit polyclonal antibody detecting Rad21 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 20 publications
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Publications
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- Nature communications 14:79472023Defining a core configuration for human centromeres during mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyantika Sen Gupta,Chris Seidel,Dai Tsuchiya,Sean McKinney,Zulin Yu,Sarah E Smith,Jay R Unruh,Jennifer L GertonPubMed 38040722
- Nature communications 14:77622023SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Qing Liu,Irene Paassen,Lars Custers,Peter Zeller,Hans Teunissen,Dilara Ayyildiz,Jiayou He,Juliane Laura Buhl,Eelco Wieger Hoving,Alexander van Oudenaarden,Elzo de Wit,Jarno DrostPubMed 38040699
- Science advances 9:eade38762023Cohesin regulates alternative splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmit K Singh,Qingrong Chen,Cu Nguyen,Daoud Meerzaman,Dinah S SingerPubMed 36857449
- Nature cell biology 24:1516-15272022CTCF-CTCF loops and intra-TAD interactions show differential dependence on cohesin ring integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Liu,Job DekkerPubMed 36202971
- Molecules and cells 45:820-8322022The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Eun Koh,Eui-Hwan Choi,Jung-Woong Kim,Keun Pil KimPubMed 36172976
- Genome biology 23:1852022CTCF and cohesin promote focal detachment of DNA from the nuclear lamina.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTom van Schaik,Ning Qing Liu,Stefano G Manzo,Daan Peric-Hupkes,Elzo de Wit,Bas van SteenselPubMed 36050765
- Nature communications 13:43422022Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Robles-Rebollo,Sergi Cuartero,Adria Canellas-Socias,Sarah Wells,Mohammad M Karimi,Elisabetta Mereu,Alexandra G Chivu,Holger Heyn,Chad Whilding,Dirk Dormann,Samuel Marguerat,Inmaculada Rioja,Rab K Prinjha,Michael P H Stumpf,Amanda G Fisher,Matthias MerkenschlagerPubMed 35896525
- Nature communications 13:21392022Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbrar Aljahani,Peng Hua,Magdalena A Karpinska,Kimberly Quililan,James O J Davies,A Marieke OudelaarPubMed 35440598
- Genome biology 23:702022Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P KimPubMed 35241136
- Methods in molecular biology (Clifton, N.J.) 2458:113-1222022A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMacarena Moronta Gines,Kerstin S WendtPubMed 35103965
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAD21 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link O60216
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Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Associated Products
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Target data
Function
Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity). 64-kDa C-terminal product. May promote apoptosis.
Alternative names
HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1
Recommended products
Anti-Rad21 antibody (ab154769) is a rabbit polyclonal antibody detecting Rad21 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAD21 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link O60216
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Rad21 antibody (ab154769) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of Rad21?
Anti-Rad21 (ab154769) specifically detects a band for Rad21 (UniProt: O60216) at a molecular weight of 72kDa.
Trusted by the scientific community
Anti-Rad21 (ab154769) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
- Biological function summary
Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.
- Pathways
Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.
- Associated diseases and disorders
Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-Rad21 antibody (ab154769)
7.5% SDS PAGE
All lanes: Western blot - Anti-Rad21 antibody (ab154769) at 1/1000 dilution
Lane 1: K562 whole cell lysate at 30 µg
Lane 2: THP1 whole cell lysate at 30 µg
Lane 3: HL60 whole cell lysate at 30 µg
Lane 4: NCI-H929 whole cell lysate at 30 µg
Predicted band size: 72 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (ab154769)
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
Western blot - Anti-Rad21 antibody (ab154769)
7.5% SDS PAGE
All lanes: Western blot - Anti-Rad21 antibody (ab154769) at 1/3000 dilution
Lane 1: NIH3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Predicted band size: 72 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
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