Anti-Rad21 antibody is a rabbit polyclonal antibody that is used to detect Rad21 in IP, Western blot. Suitable for Human, Mouse samples.
- Cited in over 200 publications
- Trusted since 2002
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- Nature communications 14:81602023Loss of cohesin regulator PDS5A reveals repressive role of Polycomb loops.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Bsteh,Hagar F Moussa,Georg Michlits,Ramesh Yelagandula,Jingkui Wang,Ulrich Elling,Oliver BellPubMed 38071364
- Nature communications 14:79472023Defining a core configuration for human centromeres during mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyantika Sen Gupta,Chris Seidel,Dai Tsuchiya,Sean McKinney,Zulin Yu,Sarah E Smith,Jay R Unruh,Jennifer L GertonPubMed 38040722
- Nature 623:183-1922023Chromatin compartmentalization regulates the response to DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColine Arnould,Vincent Rocher,Florian Saur,Aldo S Bader,Fernando Muzzopappa,Sarah Collins,Emma Lesage,Benjamin Le Bozec,Nadine Puget,Thomas Clouaire,Thomas Mangeat,Raphael Mourad,Nadav Ahituv,Daan Noordermeer,Fabian Erdel,Martin Bushell,Aline Marnef,Gaëlle LegubePubMed 37853125
- Nature communications 14:56152023Multi-feature clustering of CTCF binding creates robustness for loop extrusion blocking and Topologically Associating Domain boundaries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Hsin Chang,Sourav Ghosh,Andrea Papale,Jennifer M Luppino,Mélanie Miranda,Vincent Piras,Jéril Degrouard,Joanne Edouard,Mallory Poncelet,Nathan Lecouvreur,Sébastien Bloyer,Amélie Leforestier,Eric F Joyce,David Holcman,Daan NoordermeerPubMed 37699887
- Nature genetics 55:1164-11752023Sequential and directional insulation by conserved CTCF sites underlies the Hox timer in stembryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHocine Rekaik,Lucille Lopez-Delisle,Aurélie Hintermann,Bénédicte Mascrez,Célia Bochaton,Alexandre Mayran,Denis DuboulePubMed 37322110
- eLife 12:2023Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana HadjurPubMed 37010886
- Science advances 9:eadg11232023Light-activated macromolecular phase separation modulates transcription by reconfiguring chromatin interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoon Jung Kim,Michael Lee,Yi-Tsang Lee,Ji Jing,Jacob T Sanders,Giovanni A Botten,Lian He,Junhua Lyu,Yuannyu Zhang,Marcel Mettlen,Peter Ly,Yubin Zhou,Jian XuPubMed 37000871
- Molecular cell 83:1446-1461.e62023Structural elements promote architectural stripe formation and facilitate ultra-long-range gene regulation at a human disease locus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Fu Chen,Hannah Katherine Long,Minhee Park,Tomek Swigut,Alistair Nicol Boettiger,Joanna WysockaPubMed 36996812
- Nature microbiology 8:679-6942023SARS-CoV-2 restructures host chromatin architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoyu Wang,Joo-Hyung Lee,Jieun Kim,Feng Xiong,Lana Al Hasani,Yuqiang Shi,Erin N Simpson,Xiaoyu Zhu,Yi-Ting Chen,Pooja Shivshankar,Joanna Krakowiak,Yanyu Wang,David M Gilbert,Xiaoyi Yuan,Holger K Eltzschig,Wenbo LiPubMed 36959507
- Nature communications 14:15982023A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Wang,Xiang Liu,Jun Liang,Yohei Narita,Weiyue Ding,Difei Li,Luyao Zhang,Hongbo Wang,Merrin Man Long Leong,Isabella Hou,Catherine Gerdt,Chang Jiang,Qian Zhong,Zhonghui Tang,Carmy Forney,Leah Kottyan,Matthew T Weirauch,Benjamin E Gewurz,Mu-Sheng Zeng,Sizun Jiang,Mingxiang Teng,Bo ZhaoPubMed 36949074
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAD21 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O60216
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Turkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab992 used for IP at 6 μg per reaction. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04 µg/mL | Notes Band observed at ~130 kDa. |
Species Human | Dilution info 1/500 - 1/5000 | Notes Band observed at ~130 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity). 64-kDa C-terminal product. May promote apoptosis.
Alternative names
HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1
Recommended products
Anti-Rad21 antibody is a rabbit polyclonal antibody that is used to detect Rad21 in IP, Western blot. Suitable for Human, Mouse samples.
- Cited in over 200 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAD21 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O60216
- Purification technique
- Affinity purification Immunogen
- Specificity
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Rad21 antibody (ab992) is a rabbit polyclonal antibody and is validated for use in IP, WB in human, mouse samples.
Anti-Rad21 antibody (ab992) specifically detects Rad21 (UniProt ID: O60216; Molecular weight: 72kDa) and is sold in 50 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Rad21 antibody (ab992) has high sensitivity and specificity.
Anti-Rad21 antibody (ab992) has been cited over 207 times in peer reviewed journals and is trusted by the scientific community.
Anti-Rad21 antibody (ab992) has 21 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free ab251568.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunoprecipitation - Anti-Rad21 antibody (ab992)
Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.
Lane 1: ab992 used for IP at 6 μg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-Rad21 antibody (ab992)
Predicted band size: 72 kDa
Western blot - Anti-Rad21 antibody (ab992)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Rad21 antibody (ab992) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: RKO (human cell line from colon carcinoma) whole cell lysate at 50 µg
Lane 5: GaMG (human cell line from glioblastoma) whole cell lysate at 50 µg
Lane 6: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg
Lane 7: A549 (human lung carcinoma cell line) whole cell lysate at 50 µg
Lane 8: U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 9: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 10: TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 10s
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