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AB63800

Anti-Rad22 antibody

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(2 Publications)

Rabbit Polyclonal RAD22 antibody. Suitable for IP, ELISA, WB, ICC/IF and reacts with Schizosaccharomyces pombe samples. Cited in 2 publications.

View Alternative Names

SPAC30D11.10, rad22, DNA repair and recombination protein rad22

2 Images
Western blot - Anti-Rad22 antibody (AB63800)
  • WB

Unknown

Western blot - Anti-Rad22 antibody (AB63800)

ab63800 was used unpurified.

All lanes:

Western blot - Anti-Rad22 antibody (ab63800) at 1/2000 dilution

Lane 1:

Molecular weight markers

Lane 2:

Whole cell extract of Schizosaccharomyces pombe wild-type strain

Lane 3:

Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain

Predicted band size: 52 kDa

Observed band size: 50 kDa,60 kDa

false

Western blot - Anti-Rad22 antibody (AB63800)
  • WB

Supplier Data

Western blot - Anti-Rad22 antibody (AB63800)

All lanes:

Western blot - Anti-Rad22 antibody (ab63800)

All lanes:

S.pombe whole cell extract

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Schizosaccharomyces pombe

Applications

ICC/IF, IP, ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Schizosaccharomyces pombe": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad22 also known as RPA1 or replication protein A1 is a protein involved in DNA repair processes. It has a molecular mass of approximately 29 kDa. Rad22 is expressed in eukaryotic cells and has a critical role in maintaining the integrity of genetic material. The protein interacts with single-stranded DNA (ssDNA) during repair and replication processes forming a nucleoprotein complex that stabilizes ssDNA intermediates and protect them from nuclease degradation.
Biological function summary

Rad22 contributes to homologous recombination an essential mechanism for repairing double-strand breaks in DNA. Rad22 is a part of a larger multiprotein complex that includes Rad51 and other early recombination factors. This complex plays a role in the search for homology and the strand pairing stages of homologous recombination. Rad22's interaction with Rad51 ensures accurate repair by facilitating the invasion of the broken DNA strand into the homologous sequence.

Pathways

Cellular processes involving Rad22 engage in the homologous recombination repair pathway and DNA replication stress response. Rad22 acts synergistically with Rad51 and other recombination mediators to initiate and propagate homologous recombination. These processes ensure the correct repair of DNA to prevent genomic instability which can lead to cell cycle arrest or apoptosis.

Rad22's function impacts conditions related to genomic instability such as cancer and Bloom syndrome. Disruptions in Rad22 activity can lead to inefficient DNA repair contributing to the accumulation of mutations that drive tumorigenesis. Furthermore Rad22 interacts with other proteins like BRCA2 in tumor suppression pathways. In Bloom syndrome a disorder characterized by high rates of chromosome breakage Rad22 plays a vital part in DNA repair and any malfunctions in its activity connect to the disease phenotype.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Active in the repair of DNA damage and in mating-type switching. Probably involved in the repair of DNA double-strands breaks. Has a role in promoting S phase completion.
See full target information rad22
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 15:e1008441 PubMed31584934

2019

Histone deposition promotes recombination-dependent replication at arrested forks.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Hardy,Dingli Dai,Anissia Ait Saada,Ana Teixeira-Silva,Louise Dupoiron,Fatemeh Mojallali,Karine Fréon,Francoise Ochsenbein,Brigitte Hartmann,Sarah Lambert

Genome research 28:1179-1192 PubMed29934426

2018

The organization of genome duplication is a critical determinant of the landscape of genome maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Gómez-Escoda,Pei-Yun Jenny Wu
View all publications
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Product promise

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