Anti-Rad50 antibody [13B3/2C6]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [13B3/2C6] (AB89)

Immunohistochemical analysis of paraffin embedded pc 3 xenograft tissue stained for Rad50 protein at nucleus with ab89 at a 1/200 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rad50 antibody [13B3/2C6] (AB89)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, HeLa cells labelling Rad50 with ab89 at 1/200 dilution (green). Phalloidin at a 1/200 dilution was used to counterstain the cytoskeleton (red).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [13B3/2C6] (AB89)

ab89 (1μg/ml) staining RAD50 in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

This image was generated using the ascites version of the product.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [13B3/2C6] (AB89)

Immunohistochemical analysis of paraffin embedded human lung cancer tissue stained for Rad50 protein at nucleus with ab89 at a 1/100 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• Flow Cyt

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Flow Cytometry - Anti-Rad50 antibody [13B3/2C6] (AB89)

Overlay histogram showing HeLa cells stained with ab89 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-Rad50 antibody [13B3/2C6] (AB89)

This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.

The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)

Predicted band size: 153 kDa

false

• WB

AbReview4232****

Western blot - Anti-Rad50 antibody [13B3/2C6] (AB89)

This image is courtesy of an Abreview submitted by Philippe Szankasi on 24 February 2006.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89) at 1/1000 dilution

All lanes:

50 ug Human HEK293 total cell lysate

Secondary

All lanes:

HRP conjugated Donkey Anti-Mouse IgG

Predicted band size: 153 kDa,57 kDa,68 kDa

Observed band size: 100 kDa,160 kDa,300 kDa,68 kDa

true

Exposure time: 30s