Rabbit Polyclonal RAD50 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAD50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAD50. The exact immunogen used to generate this antibody is proprietary information. Database link Q92878
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:15064416, PubMed:21757780, PubMed:27889449, PubMed:28134932, PubMed:28867292, PubMed:9590181, PubMed:9651580, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:15064416, PubMed:21757780, PubMed:27889449, PubMed:28867292, PubMed:9590181, PubMed:9651580, PubMed:9705271). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:15064416, PubMed:27889449, PubMed:28867292, PubMed:9590181, PubMed:9651580, PubMed:9705271). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (PubMed:11741547, PubMed:9590181, PubMed:9651580, PubMed:9705271). Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11 (PubMed:11741547, PubMed:12805565, PubMed:28134932). RAD50 provides an ATP-dependent control of MRE11 by positioning DNA ends into the MRE11 active site: ATP-binding induces a large structural change from an open form with accessible MRE11 nuclease sites into a closed form (By similarity). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR (PubMed:15064416, PubMed:15790808, PubMed:16622404). The MRN complex is also required for the processing of R-loops (PubMed:31537797). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
Alternative names
DNA repair protein RAD50, hRAD50, RAD50
Recommended products
Rabbit Polyclonal RAD50 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAD50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAD50. The exact immunogen used to generate this antibody is proprietary information. Database link Q92878
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad50 is an important component of the MRN complex with its partners MRE11 and NBS1. This protein plays an important role in DNA double-strand break repair and telomere maintenance. Rad50 also known by its molecular weight of approximately 153 kDa possesses ATPase activity that facilitates the bridging of DNA ends during repair processes. It is expressed in a variety of tissues with higher levels observed in rapidly dividing cells such as those found in testes and lymphoid organs.
- Biological function summary
Rad50 contributes to genomic stability by participating in non-homologous end joining (NHEJ) and homologous recombination (HR) both of which are DNA repair mechanisms. It is part of the MRN complex a multi-protein assembly that detects DNA breaks and aids in processing and signaling them for repair. This complex acts at the early stages of DNA damage response assisting in the recruitment of other repair enzymes and proteins to the site of DNA lesions.
- Pathways
Rad50 is integral to DNA damage response signaling and repair pathways like Ataxia Telangiectasia Mutated (ATM) signaling. The MRN complex activates ATM a kinase that phosphorylates key substrates involved in controlling cell cycle checkpoints and promoting repair of damaged DNA. This relationship positions Rad50 and its complex members as significant participants in maintaining cellular integrity by preventing the accumulation of genetic mutations.
- Associated diseases and disorders
Rad50 mutations have links to cancer development due to their effect on genomic instability. Deficiencies in Rad50 or the MRN complex can impair DNA repair leading to an increased risk for neoplastic transformations. Additionally Rad50 interactions with proteins such as p53 a tumor suppressor underline its potential role in the mechanisms behind cancers like breast and ovarian cancer. These connections highlight the importance of Rad50 in understanding disease progression and developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-Rad50 antibody - N-terminal (ab228935)
5% SDS-PAGE
All lanes: Western blot - Anti-Rad50 antibody - N-terminal (ab228935) at 1/4000 dilution
Lane 1: Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
Lane 2: Rad50 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 153 kDa
Immunoprecipitation - Anti-Rad50 antibody - N-terminal (ab228935)
Rad50 was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate with 4 μg ab228935. Western blot was performed from the immunoprecipitate using ab228935 at a 1/500 dilution.
Lane 1: 50 μg NT2/D1 whole cell lysate.
Lane 2: Control with 4 μg preimmune Rabbit IgG.
Lane 3: ab228935 in NT2/D1 whole cell lysate.
5% SDS-PAGE
All lanes: Immunoprecipitation - Anti-Rad50 antibody - N-terminal (ab228935)
Predicted band size: 153 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody - N-terminal (ab228935)
Paraffin-embedded human lung carcinoma tissue stained for Rad50 with ab228935 at a 1/250 dilution in immunohistochemical analysis.
Western blot - Anti-Rad50 antibody - N-terminal (ab228935)
5% SDS-PAGE
All lanes: Western blot - Anti-Rad50 antibody - N-terminal (ab228935) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 153 kDa
Western blot - Anti-Rad50 antibody - N-terminal (ab228935)
5% SDS-PAGE
All lanes: Western blot - Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: GL261 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 153 kDa
Western blot - Anti-Rad50 antibody - N-terminal (ab228935)
5% SDS-PAGE
All lanes: Western blot - Anti-Rad50 antibody - N-terminal (ab228935) at 1/5000 dilution
All lanes: NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 153 kDa
Western blot - Anti-Rad50 antibody - N-terminal (ab228935)
5% SDS-PAGE
All lanes: Western blot - Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution
All lanes: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 153 kDa
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