Mouse Recombinant Monoclonal Rad51 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 84 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51.
Images
![Western blot - Anti-Rad51 antibody [14B4] (AB213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img85245.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rad51 antibody [14B4] (AB213)")
![Western blot - Anti-Rad51 antibody [14B4] (AB213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img17890.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rad51 antibody [14B4] (AB213)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (AB213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114334.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (AB213)")
![Western blot - Anti-Rad51 antibody [14B4] (AB213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img16099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rad51 antibody [14B4] (AB213)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (AB213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125577.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (AB213)")
Publications
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- Nature communications 14:44452023RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael A Longo,Sunetra Roy,Yue Chen,Karl-Heinz Tomaszowski,Andrew S Arvai,Jordan T Pepper,Rebecca A Boisvert,Selvi Kunnimalaiyaan,Caezanne Keshvani,David Schild,Albino Bacolla,Gareth J Williams,John A Tainer,Katharina SchlacherPubMed 37488098
- Nature communications 13:57812022XPF activates break-induced telomere synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Yu Guh,Hong-Jhih Shen,Liv WeiChien Chen,Pei-Chen Chiu,I-Hsin Liao,Chen-Chia Lo,Yunfei Chen,Yu-Hung Hsieh,Ting-Chia Chang,Chien-Ping Yen,Yi-Yun Chen,Tom Wei-Wu Chen,Liuh-Yow Chen,Ching-Shyi Wu,Jean-Marc Egly,Hsueh-Ping Catherine ChuPubMed 36184605
- iScience 25:1041422022Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharmistha Chakraborty,Mayank Singh,Raj K Pandita,Vipin Singh,Calvin S C Lo,Fransisca Leonard,Nobuo Horikoshi,Eduardo G Moros,Deblina Guha,Clayton R Hunt,Eric Chau,Kazi M Ahmed,Prayas Sethi,Vijaya Charaka,Biana Godin,Kalpana Makhijani,Harry Scherthan,Jeanette Deck,Michael Hausmann,Arjamand Mushtaq,Mohammad Altaf,Kenneth S Ramos,Krishna M Bhat,Nitika Taneja,Chandrima Das,Tej K PanditaPubMed 35434547
- Communications biology 5:2512022Pre-activation of autophagy impacts response to olaparib in prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxime Cahuzac,Patricia Langlois,Benjamin Péant,Hubert Fleury,Anne-Marie Mes-Masson,Fred SaadPubMed 35318456
- Molecular cell 81:3007-3017.e52021RADX prevents genome instability by confining replication fork reversal to stalled forks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArchana Krishnamoorthy,Jessica Jackson,Taha Mohamed,Madison Adolph,Alessandro Vindigni,David CortezPubMed 34107305
- Genes 12:2021MET Expression and Cancer Stem Cell Networks Impact Outcome in High-Grade Serous Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Bååth,Jenny-Maria Jönsson,Sofia Westbom Fremer,Laura Martín de la Fuente,Lena Tran,Susanne Malander,Päivi Kannisto,Anna Måsbäck,Gabriella Honeth,Ingrid HedenfalkPubMed 34069138
- Nature 592:799-8032021AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmiliano Maiani,Giacomo Milletti,Francesca Nazio,Søs Grønbæk Holdgaard,Jirina Bartkova,Salvatore Rizza,Valentina Cianfanelli,Mar Lorente,Daniele Simoneschi,Miriam Di Marco,Pasquale D'Acunzo,Luca Di Leo,Rikke Rasmussen,Costanza Montagna,Marilena Raciti,Cristiano De Stefanis,Estibaliz Gabicagogeascoa,Gergely Rona,Nélida Salvador,Emanuela Pupo,Joanna Maria Merchut-Maya,Colin J Daniel,Marianna Carinci,Valeriana Cesarini,Alfie O'sullivan,Yeon-Tae Jeong,Matteo Bordi,Francesco Russo,Silvia Campello,Angela Gallo,Giuseppe Filomeni,Letizia Lanzetti,Rosalie C Sears,Petra Hamerlik,Armando Bartolazzi,Robert E Hynds,David R Pearce,Charles Swanton,Michele Pagano,Guillermo Velasco,Elena Papaleo,Daniela De Zio,Apolinar Maya-Mendoza,Franco Locatelli,Jiri Bartek,Francesco CecconiPubMed 33854232
- Human cell 34:1163-11732021LINC00200 contributes to the chemoresistance to oxaliplatin of gastric cancer cells via regulating E2F1/RAD51 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxin Lin,Meifang Xu,Zongbin Xu,Zongqi Weng,Bingqiang Lin,Yanqin Lan,Qing Liu,Xiaoyan Lin,Jie PanPubMed 33822325
- Molecular cell 81:1074-1083.e52021RADX controls RAD51 filament dynamics to regulate replication fork stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadison B Adolph,Taha M Mohamed,Swati Balakrishnan,Chaoyou Xue,Florian Morati,Mauro Modesti,Eric C Greene,Walter J Chazin,David CortezPubMed 33453169
- Cancers 12:2020RAD51-Mediated DNA Homologous Recombination Is Independent of Mutational Status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsha Sinha et. al.PubMed 33138032
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes Please see WB protocol in image legend. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:22325354, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658, PubMed:32640219). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658, PubMed:38459011). Recruited to resolve stalled replication forks during replication stress (PubMed:27797818, PubMed:31844045). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR (PubMed:12442171, PubMed:24141787). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (PubMed:20413593). Also involved in interstrand cross-link repair (PubMed:26253028).
Alternative names
RAD51A, RECA, RAD51A, RAD51, RECA, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A
Recommended products
Mouse Recombinant Monoclonal Rad51 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 84 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
- Carrier free
Yes
- Clone number
14B4
- Purification technique
Affinity purification Immunogen
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The production method has changed from hybridoma to recombinant on 22nd March 2024
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
- Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
- Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
- Associated diseases and disorders
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img85245.jpg "Western blot - Anti-Rad51 antibody [14B4] (ab213)")
Image courtesy of an anonymous customer review.Western blot - Anti-Rad51 antibody [14B4] (ab213)
All lanes: Western blot - Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
All lanes: lysate prepared from murine NIH3T3 cells at 20 µg
SecondaryAll lanes: HRP conjugated anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 42 kDa, 45 kDa
Exposure time: 5min
![Western blot - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img17890.jpg "Western blot - Anti-Rad51 antibody [14B4] (ab213)")
Western blot - Anti-Rad51 antibody [14B4] (ab213)
10% SDS-PAGE
Running condition: 80V, 15min; 140V, 40 min
Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60 min.
Primary antibody incubation: 4°C overnight.
Washing condition: 5 ml TBST, 4 x 5 min.
Chemiluminescent HRP Substrate.
All lanes: Western blot - Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114334.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
![Western blot - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--western-blot-img16099.jpg "Western blot - Anti-Rad51 antibody [14B4] (ab213)")
Western blot - Anti-Rad51 antibody [14B4] (ab213)
10% SDS-PAGE
All lanes: Western blot - Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1: Non-transfected 293T (-) whole cell extracts at 30 µg
Lane 2: RAD51 shRNA transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125577.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84905.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138323.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunocytochemistry-immunofluorescence-img428896.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [14B4] (ab213)")
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [14B4] (ab213)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed Mock and treated HeLa cells labelling Rad51 with ab213 at 1/500 dilution (Green). Blue: Fluoroshield with DAPI
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84905.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138323.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)")
![Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [14B4] (ab213), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-14b4-ab213--immunocytochemistry-immunofluorescence-img428896.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [14B4] (ab213)")
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