AB1837

Anti-Rad51 antibody [51RAD01]

Name: Anti-Rad51 antibody [51RAD01] (ab1837) | Abcam
Brand: Abcam Limited
SKU: AB1837
Rating: 3 (9 reviews)

(9 Reviews)

(21 Publications)

Mouse Monoclonal Rad51 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 21 publications.

View Alternative Names

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

1 Images

Flow Cytometry - Anti-Rad51 antibody [51RAD01] (AB1837)

Flow Cyt

Unknown

Flow Cytometry - Anti-Rad51 antibody [51RAD01] (AB1837)

Overlay histogram showing HeLa cells stained with ab1837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1837, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

51RAD01

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>This antibody gives inconsistent results on tissue sections in IHC staining and we cannot guarantee it for IHC-P.</p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.

Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically.

See full target information RAD51

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:234 PubMed37684630

2023

SENP5 promotes homologous recombination-mediated DNA damage repair in colorectal cancer cells through H2AZ deSUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Liu,Hang Wang,Yuanyuan Chen,Zhijie Wan,Zhipeng Du,Hui Shen,Yue Yu,Shengzhe Ma,Ying Xu,Zhuqing Li,Nanxi Yu,Fangxiao Zhang,Kun Cao,Jianming Cai,Wei Zhang,Fu Gao,Yanyong Yang

Redox biology 64:102757 PubMed37285741

2023

A superoxide-driven redox state promotes geroconversion and resistance to senolysis in replication-stress associated senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Le Luo,Shazib Pervaiz,Marie-Veronique Clement

PLoS pathogens 18:e1010275 PubMed35148356

2022

Beta human papillomavirus 8 E6 allows colocalization of non-homologous end joining and homologous recombination repair factors.

Applications

Unspecified application

Species

Unspecified reactive species

Changkun Hu,Taylor Bugbee,Dalton Dacus,Rachel Palinski,Nicholas Wallace

Cell reports 34:108840 PubMed33730584

2021

DSB repair pathway choice is regulated by recruitment of 53BP1 through cell cycle-dependent regulation of Sp1.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle L Swift,Kate Beishline,Samuel Flashner,Jane Azizkhan-Clifford

Epigenetics & chromatin 12:53 PubMed31493790

2019

TH2BS11ph histone mark is enriched in the unsynapsed axes of the XY body and predominantly associates with H3K4me3-containing genomic regions in mammalian spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Iyer Aditya Mahadevan,Satyakrishna Pentakota,Raktim Roy,Utsa Bhaduri,Manchanahalli R Satyanarayana Rao

The Journal of clinical investigation 129:786-801 PubMed30480549

2019

Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sahra Bodo,Cécile Campagne,Tin Htwe Thin,Daniel S Higginson,H Alberto Vargas,Guoqiang Hua,John D Fuller,Ellen Ackerstaff,James Russell,Zhigang Zhang,Stefan Klingler,HyungJoon Cho,Matthew G Kaag,Yousef Mazaheri,Andreas Rimner,Katia Manova-Todorova,Boris Epel,Joan Zatcky,Cristian R Cleary,Shyam S Rao,Yoshiya Yamada,Michael J Zelefsky,Howard J Halpern,Jason A Koutcher,Carlos Cordon-Cardo,Carlo Greco,Adriana Haimovitz-Friedman,Evis Sala,Simon N Powell,Richard Kolesnick,Zvi Fuks

Nucleic acids research 45:10564-10582 PubMed28985345

2017

Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Gireedhar Venkatachalam,Uttam Surana,Marie-Véronique Clément

Nature 543:205-210 PubMed28241143

2017

Autophagy maintains the metabolism and function of young and old stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Theodore T Ho,Matthew R Warr,Emmalee R Adelman,Olivia M Lansinger,Johanna Flach,Evgenia V Verovskaya,Maria E Figueroa,Emmanuelle Passegué

Biology of reproduction 96:491-501 PubMed28203708

2017

Obesity alters phosphoramide mustard-induced ovarian DNA repair in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shanthi Ganesan,Jackson Nteeba,Jill A Madden,Aileen F Keating

Nature communications 6:5931 PubMed25585578

2015

FBH1 influences DNA replication fork stability and homologous recombination through ubiquitylation of RAD51.

Applications

Unspecified application

Species

Unspecified reactive species

Wai Kit Chu,Miranda J Payne,Petra Beli,Katsuhiro Hanada,Chunaram Choudhary,Ian D Hickson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rad51 antibody [51RAD01]

Flow Cytometry - Anti-Rad51 antibody [51RAD01] (AB1837)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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