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AB176458

Anti-Rad51 antibody

5

(4 Reviews)

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(32 Publications)

Anti-Rad51 antibody (ab176458) is a rabbit polyclonal antibody detecting Rad51 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Chinese hamster, Human, Mouse, Xenopus laevis.

- Over 20 publications

View Alternative Names

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

5 Images
Western blot - Anti-Rad51 antibody (AB176458)
  • WB

Supplier Data

Western blot - Anti-Rad51 antibody (AB176458)

All lanes:

Western blot - Anti-Rad51 antibody (ab176458) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 40 µg

Lane 2:

NIH3T3 (Mouse embryo fibroblast cell line) cell lysate at 40 µg

Lane 3:

CHO (Chinese hamster ovary cell line) cell lysate at 40 µg

Lane 4:

Xenopus laevis egg at 40 µg

Predicted band size: 36 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB176458)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB176458)

Immunofluorescence detection of Rad51 foci formation after X-ray irradiation in GM0637 cells with ab176458 at 1/10000 dilution (left panels) and 1/1000 dilution (right panels). The secondary antibody, anti-rabbit Alexa 488 was used at 1/10000 dilution.

Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with ab176458 for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted.

The pictures were by courtesy of Prof. S. Tashiro and Dr. K. Kono at Hiroshima University.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody (AB176458)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody (AB176458)

Immunohistological staining of Rad51 protein in mouse testis using ab176458. A section of formalin fixed and paraffin embedded mouse testis was treated with ab176458 at 1/100 dilution after deparaffization and antigen retrieval. The secondary antibody, Alexa Fluor® 647conjugated anti-rabbit IgG was used at 1/1,000 dilution (top left). The sample was counter-stained with DAPI (top right) and the merged image is shown (bottom left). The white light image of the same region is shown on the bottom right.

Western blot - Anti-Rad51 antibody (AB176458)
  • WB

Supplier Data

Western blot - Anti-Rad51 antibody (AB176458)

All lanes:

Western blot - Anti-Rad51 antibody (ab176458) at 1/1000 dilution

All lanes:

Crude HeLa cell extracts at 10 µg

Secondary

All lanes:

Goat anti-Rabbit IgG conjugated to HRP at 1/20000 dilution

Predicted band size: 36 kDa

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Immunoprecipitation - Anti-Rad51 antibody (AB176458)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rad51 antibody (AB176458)

ab176458 (20 μg) was incubated with 20 μg of HeLa cell extract, and precipitated with 20 μg of proteinA-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with anti-Rad51 antiserum (chicken, ab63802) at 1/1000 dilution. As secondary antibody, anti-chicken IgG antibody (rabbit) was used at 1/10000 dilution.

All lanes:

Immunoprecipitation - Anti-Rad51 antibody (ab176458)

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Xenopus laevis, Chinese hamster

Applications

WB, IP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAD51.

Q06609

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Rad51 antibody (ab176458) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Chinese hamster, Human, Mouse, Xenopus laevis samples.

What is the molecular weight of Rad51?
Anti-Rad51 (ab176458) specifically detects a band for Rad51 (UniProt: Q06609) at a molecular weight of 36kDa.

Trusted by the scientific community
Anti-Rad51 (ab176458) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically.
See full target information RAD51
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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115259 PubMed39893637

2025

The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Graham,Lucia Rampazzo,Chin Wei Brian Leung,Jacob Wall,Emőke Zsanett Gerőcz,Mikhail Liskovykh,Nikolay Goncharov,Xanita Saayman,Ramazan Gundogdu,Masato T Kanemaki,Hiroshi Masumoto,Vladimir Larionov,Natalay Kouprina,Fumiko Esashi

Nature communications 15:9463 PubMed39487138

2024

Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Sun,Nourhan Nashat Ali,Daniela Londoño-Vásquez,Constantine A Simintiras,Huanyu Qiao,M Sofia Ortega,Yuksel Agca,Masashi Takahashi,Rocío M Rivera,Andrew M Kelleher,Peter Sutovsky,Amanda L Patterson,Ahmed Z Balboula

iScience 27:110180 PubMed38993666

2024

A high-throughput approach to identify BRCA1-downregulating compounds to enhance PARP inhibitor sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Erin Sellars,Margarita Savguira,Jie Wu,Sabrina Cancelliere,Mark Jen,Rehna Krishnan,Anne Hakem,Dalia Barsyte-Lovejoy,Razqallah Hakem,Steven A Narod,Joanne Kotsopoulos,Leonardo Salmena

Science (New York, N.Y.) 385:eado3867 PubMed38900911

2024

Structure and repair of replication-coupled DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Pavani,Veenu Tripathi,Kyle B Vrtis,Dali Zong,Raj Chari,Elsa Callen,Ajith V Pankajam,Gang Zhen,Gabriel Matos-Rodrigues,Jiajie Yang,Shuheng Wu,Giordano Reginato,Wei Wu,Petr Cejka,Johannes C Walter,André Nussenzweig

Cell proliferation 57:e13685 PubMed38894566

2024

MRE11 is essential for the long-term viability of undifferentiated spermatogonia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenghui Tang,Zhongyang Liang,Bin Zhang,Xiaohui Xu,Peng Li,Lejun Li,Lin-Yu Lu,Yidan Liu

International journal of molecular medicine 52: PubMed37888610

2023

Exosomes derived from human dermal fibroblasts protect against UVB‑induced skin photoaging.

Applications

Unspecified application

Species

Unspecified reactive species

A Yeon Park,Jung Ok Lee,Youna Jang,Yu-Jin Kim,Jung Min Lee,Su-Young Kim,Beom Joon Kim,Kwang Ho Yoo

Science advances 9:eadg0898 PubMed37862420

2023

CHEK2 signaling is the key regulator of oocyte survival after chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Chihiro Emori,Zachary Boucher,Ewelina Bolcun-Filas

Nature communications 14:1838 PubMed37012254

2023

Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Zhang,Mingzhu Wang,Zhiwei Song,Qianzheng Fu,Jiayu Chen,Weitao Zhang,Shuai Gao,Xiaoxiang Sun,Guang Yang,Qiang Zhang,Jiaqing Yang,Huanyin Tang,Haiyan Wang,Xiaochen Kou,Hong Wang,Zhiyong Mao,Xiaojun Xu,Shaorong Gao,Ying Jiang

Molecular cell 83:1043-1060.e10 PubMed36854302

2023

SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhou Huang,Chenming Wu,Jake A Kloeber,Huanyao Gao,Ming Gao,Qian Zhu,Yiming Chang,Fei Zhao,Guijie Guo,Kuntian Luo,Haiming Dai,Sijia Liu,Qiru Huang,Wootae Kim,Qin Zhou,Shouhai Zhu,Zheming Wu,Xinyi Tu,Ping Yin,Min Deng,Liewei Wang,Jian Yuan,Zhenkun Lou

Molecular cell 83:523-538.e7 PubMed36702125

2023

Centromeres as universal hotspots of DNA breakage, driving RAD51-mediated recombination during quiescence.

Applications

Unspecified application

Species

Unspecified reactive species

Xanita Saayman,Emily Graham,William J Nathan,Andre Nussenzweig,Fumiko Esashi
View all publications
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