Anti-Rad51 antibody (ab63801) is a rabbit polyclonal antibody that is used to detect Rad51 in Western Blot, ICC/IF. Suitable for Human, Xenopus laevis samples.
- Over 130 publications
- Trusted since 2008
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- Science advances 9:eadi66812023RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Zhang,Yun Xiong,Yilun Sun,Jeong-Min Park,Dan Su,Xu Feng,Sarah Keast,Mengfan Tang,Min Huang,Chao Wang,Mrinal Srivastava,Chang Yang,Dandan Zhu,Zhen Chen,Siting Li,Ling Yin,Yves Pommier,Junjie ChenPubMed 38055811
- Nature communications 14:78822023CaMKK2 and CHK1 phosphorylate human STN1 in response to replication stress to protect stalled forks from aberrant resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRishi Kumar Jaiswal,Kai-Hang Lei,Megan Chastain,Yuan Wang,Olga Shiva,Shan Li,Zhongsheng You,Peter Chi,Weihang ChaiPubMed 38036565
- International journal of molecular sciences 24:2023Arsenite Impairs BRCA1-Dependent DNA Double-Strand Break Repair, a Mechanism Potentially Contributing to Genomic Instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTizia Matthäus,Sandra Stößer,Hatice Yasemin Seren,Vivien M M Haberland,Andrea HartwigPubMed 37762697
- International journal of molecular sciences 24:2023CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole L Batenburg,John R Walker,Xu-Dong ZhuPubMed 37569794
- Journal of cellular physiology 238:1836-18492023Carbon ions trigger DNA damage response to overcome radioresistance by regulating β-catenin signaling in quiescent HeLa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Zhang,Yi Xie,Xiaoyi Liu,Lu Gan,Pingping Li,Zhihui Dou,Cuixia Di,Hong Zhang,Jing SiPubMed 37334439
- Acta pharmaceutica Sinica. B 13:3382-33992023Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1-BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Yue,Tingyu Liu,Xuecen Wang,Weijian Wu,Gesi Wen,Yang Yi,Jiaxin Wu,Ziyang Wang,Weixiang Zhan,Ruirui Wu,Yuan Meng,Zhirui Cao,Liyuan Le,Wenyan Qiu,Xiaoyue Zhang,Zhenyu Li,Yong Chen,Guohui Wan,Xianzhang Bu,Zhenwei Peng,Ran-Yi LiuPubMed 37655321
- Biomaterials science 11:2912-29232023Melatonin loaded PLGA nanoparticles effectively ameliorate the maturation of deteriorated oocytes and the cryoprotective abilities during vitrification process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujin Lee,Hye Jin Kim,Hui Bang Cho,Hye-Ryoung Kim,Sujeong Lee,Ji-In Park,Keun-Hong ParkPubMed 36883517
- EMBO molecular medicine 15:e162352023Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Min Wang,Pingyuan Wang,Xiao-Min Chen,Hui Yang,Shan-Shan Song,Zilan Song,Li Jia,Hua-Dong Chen,Xu-Bin Bao,Ne Guo,Xia-Juan Huan,Yong Xi,Yan-Yan Shen,Xin-Ying Yang,Yi Su,Yi-Ming Sun,Ying-Lei Gao,Yi Chen,Jian Ding,Jing-Yu Lang,Ze-Hong Miao,Ao Zhang,Jin-Xue HePubMed 36652375
- Nature structural & molecular biology 30:115-1242023Replication fork uncoupling causes nascent strand degradation and fork reversal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamar Kavlashvili,Wenpeng Liu,Taha M Mohamed,David Cortez,James M DewarPubMed 36593312
- International journal of molecular sciences 23:2022Quercetin and Isorhamnetin Reduce Benzo[a]pyrene-Induced Genotoxicity by Inducing RAD51 Expression through Downregulation of miR-34a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Kim,Seung-Cheol Jee,Min-Kyoung Shin,Dong-Hee Han,Kyung-Bin Bu,Seung-Cheol Lee,Bo-Young Jang,Jung-Suk SungPubMed 36361910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.5 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Xenopus laevis | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info 1/2000 - 1/10000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A
Recommended products
Anti-Rad51 antibody (ab63801) is a rabbit polyclonal antibody that is used to detect Rad51 in Western Blot, ICC/IF. Suitable for Human, Xenopus laevis samples.
- Over 130 publications
- Trusted since 2008
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.5 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Rad51 antibody (ab63801) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Xenopus laevis samples.
What is the molecular weight of Rad51?
Anti-Rad51 (ab63801) specifically detects a band for Rad51 (UniProt: Q06609) at a molecular weight of 37kDa.
Trusted by the scientific community
Anti-Rad51 (ab63801) was first used in a scientific publication in 2008 and has been cited over 130 times in peer-reviewed journals.
Reviewed by scientists
Anti-Rad51 (ab63801) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
- Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
- Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
- Associated diseases and disorders
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-Rad51 antibody (ab63801)
All lanes: Western blot - Anti-Rad51 antibody (ab63801) at 1/1000 dilution
All lanes: Crude extract of Xenopus
Predicted band size: 36 kDa
Image from PLoS Genet. 2013 Aug; 9(8): e1003667. Fig 1B, doi: 10.1371/journal.pgen.1003667 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (ab63801)
2BN hTert (XLF-deficient) human fibroblasts were fixed for 30 min with methanol at −20°C, dipped for 1 min in ice cold acetone for permeabilization and washed three times for 10 min with PBS/1% FCS. Non-specific antigens were blocked for 30 min in 5% BSA in PBS/1% FCS. Samples were incubated with ab63801 (1∶15000) in PBS/1% FCS over night at 4°C, washed three times in PBS/1% FCS and incubated for 1 h at room temperature with AlexaFluor 488 conjugated secondary antibodies (1 : 500). After three times of washing in PBS, cells were DAPI stained.
Image shows 2BN hTert (XLF-deficient) human fibroblasts analyzed 2 h post IR with 1 Gy.
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (ab63801)
Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma U2OS cells X-ray irradiation before and 2h after, immunostained with Anti-Rad51 antibody (ab63801) at 1/6,000 dilution. The lower panels were the same cells with Hoechst.
Western blot - Anti-Rad51 antibody (ab63801)
All lanes: Western blot - Anti-Rad51 antibody (ab63801) at 1/2000 dilution
Lane 1: Molecular weight markers
Lane 2: HeLa cell extract
Predicted band size: 36 kDa
Observed band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (ab63801)
Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801
(x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
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