Anti-Rad51 antibody

• WB

Supplier Data

Western blot - Anti-Rad51 antibody (AB63801)

All lanes:

Western blot - Anti-Rad51 antibody (ab63801) at 1/1000 dilution

All lanes:

Crude extract of Xenopus

Predicted band size: 36 kDa

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• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB63801)

Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801 (x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB63801)

Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma U2OS cells X-ray irradiation before and 2h after, immunostained with Anti-Rad51 antibody (ab63801) at 1/6,000 dilution. The lower panels were the same cells with Hoechst.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB63801)

2BN hTert (XLF-deficient) human fibroblasts were fixed for 30 min with methanol at −20°C, dipped for 1 min in ice cold acetone for permeabilization and washed three times for 10 min with PBS/1% FCS. Non-specific antigens were blocked for 30 min in 5% BSA in PBS/1% FCS. Samples were incubated with ab63801 (1∶15000) in PBS/1% FCS over night at 4°C, washed three times in PBS/1% FCS and incubated for 1 h at room temperature with AlexaFluor 488 conjugated secondary antibodies (1  :  500). After three times of washing in PBS, cells were DAPI stained.

Image shows 2BN hTert (XLF-deficient) human fibroblasts analyzed 2 h post IR with 1 Gy.

Image from PLoS Genet. 2013 Aug; 9(8): e1003667. Fig 1B, doi: 10.1371/journal.pgen.1003667 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Unknown

Western blot - Anti-Rad51 antibody (AB63801)

All lanes:

Western blot - Anti-Rad51 antibody (ab63801) at 1/2000 dilution

Lane 1:

Molecular weight markers

Lane 2:

HeLa cell extract

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody (AB63801)

Immunocytochemistry-immunofluorescence using Anti-Rad51 antibody, ab63801. Publication image from Stilgenbauer, S. et al., 2018, Nat Commun, 29449575. Legend direct from paper.

TCL1A affects ATM functions and cooperates with ATM deficiency. a Protracted TCL1A overexpression in HH T-cell leukemia cells mediates telomere shortening (flow-FISH; Supplementary Fig. 17b–d for controls and qRT-PCR based validations, mean with SEM; Student's t-test). b H2DCFDA-based measurements of ROS induction upon γ-irradiation (10 Gy) comparing parental Hut78 T-cell leukemia cells to their derivatives of stable TCL1A transfection. Left : examples of H2DCFDA fluorescence readout 48 h post irradiation, one representative experiment shown. Right : quantification of mean fluorescence intensity (MFI, n = 3 biological replicates, mean with SEM) relative to Hut78 cells without irradiation (p = 0.017, Student's t-test). c Enforced TCL1A expression in HH cells (doxycycline-inducible iHH) mediates higher peak focus counts of DSB marks (γH2AX, RAD51, and TP53BP1) and their impaired resolution in time-lines after washout from a preceding 1 h of Etoposide exposure. Left : quantified focus counts (mean with SEM; Student's t-test, ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05); right : representative examples (scale bar = 7.5 µm; controls in Supplementary Fig. 17f–h). d Left : setup of MEFs (Rosa-CreERT2;ATMfl/wt and Rosa-CreERT2;ATMfl/KD)76 stably transfected to express human (h)TCL1A. ATM loss was induced through Tamoxifen treatment over 3 passages resulting in ATM−/wt and ATM−/KD cells. Immunoblots in Supplementary Fig. 18a verify reduced ATM protein and overexpression of TCL1A. The KD-mutations D2880A/N2885K correspond to D2870A/N2875K in human ATM. Right : γ-irradiation reduced cell viability (proliferation) as per MTT assay (48 h) with TCL1A mediating a protective effect in scenarios of genetic ATM disruption (mean with SEM; Student's t-tests). e In vivo model of overexpression of hTCL1A and inducible ATM-abrogation. Left : hematopoetic stem cells (HSCs) of Rosa-CreERT2;ATMfl/fl mice were retrovirally transduced with hTCL1A or a GFP control vector and transplanted into irradiated syngeneic hosts. Recombination of the ATM locus (fl/fl) was induced by Tamoxifen (TM) injections starting 12 weeks after transplantation (1 mg/day i.p. for 5 consecutive days; further details in Supplementary Fig. 18c, d and Methods section). Middle : Kaplan–Meier curve showing accelerated T-cell lymphoma/leukemia onset and shorter animal survival of the ATMfl/fl/hTCL1Atg genotype (log-rank tests, time from transplantation to event). Right : evidence of hTCL1A-protein positive T-cells (flow cytometry) in blood and thymus involved by a CD4+/8+ T-cell tumor (ATMfl/fl/hTCL1Atg). H/E stains (scale bar left = 100 µm; right = 400 µm) of one exemplary thymic T-cell lymphoma (ATMfl/fl/hTCL1Atg)