Mouse Polyclonal Rad51 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 55 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51.
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- Cell death discovery 9:3852023YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng-Ai Chang,Rui-Zhi Ou Yang,Jing-Ming Su,Thi My Hang Nguyen,Junne-Ming Sung,Ming-Jer Tang,Wen-Tai ChiuPubMed 37863897
- Frontiers in oncology 12:9257182022The BET Inhibitor JQ1 Potentiates the Anticlonogenic Effect of Radiation in Pancreatic Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick L Garcia,Aubrey L Miller,Ling Zeng,Robert C A M van Waardenburg,Eddy S Yang,Karina J YoonPubMed 35795040
- Journal of cellular and molecular medicine 26:776-7882021Studying the mechanism of sperm DNA damage caused by folate deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Meilin Peng,Hongfang Yuan,Chunyan Liu,Yuan Zhang,Yiwei Fang,Yufang Su,Xinzong Zhang,Huiping Zhang,Yunge Tang,Kai ZhaoPubMed 34953021
- Science advances 7:eabg82052021RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Zhang,Nadine Übelmesser,Natasa Josipovic,Giada Forte,Johan A Slotman,Michael Chiang,Henrike Johanna Gothe,Eduardo Gade Gusmao,Christian Becker,Janine Altmüller,Adriaan B Houtsmuller,Vassilis Roukos,Kerstin S Wendt,Davide Marenduzzo,Argyris PapantonisPubMed 34678064
- Neoplasma 68:1310-13192021Expression and prognostic significance of the DNA damage response pathway and autophagy markers in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Bo Xu,Nian-Shuang Li,Huan Wang,Yi Hu,Xi-Dong Wu,Jun-Bo Hong,Nong-Hua Lu,Chuan XiePubMed 34689566
- Science advances 7:2021ATM-phosphorylated SPOP contributes to 53BP1 exclusion from chromatin during DNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDejie Wang,Jian Ma,Maria Victoria Botuyan,Gaofeng Cui,Yuqian Yan,Donglin Ding,Yingke Zhou,Eugene W Krueger,Jiang Pei,Xiaosheng Wu,Liguo Wang,Huadong Pei,Mark A McNiven,Dingwei Ye,Georges Mer,Haojie HuangPubMed 34144977
- Molecules (Basel, Switzerland) 26:2021Production of Pyracantha Polysaccharide-Iron(III) Complex and Its Biologic Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan-Fen Li,Hao-Hai Ma,Shuai Yuan,Xi-Feng ZhangPubMed 33808420
- Aging cell 20:e133072021Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeina Zhang,Jiaqing Yang,Yu Chen,Renhao Xue,Zhiyong Mao,Wen Lu,Ying JiangPubMed 33455051
- Cancer cell 38:844-856.e72020Elevated CXorf67 Expression in PFA Ependymomas Suppresses DNA Repair and Sensitizes to PARP Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJichang Han,Meng Yu,Yiqin Bai,Jianzhong Yu,Fei Jin,Chen Li,Rong Zeng,Jinghong Peng,Ao Li,Xiaomin Song,Hao Li,Dianqing Wu,Lin LiPubMed 33186520
- eLife 9:2020ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Wells,Emmanuelle Bitoun,Daniela Moralli,Gang Zhang,Anjali Hinch,Julia Jankowska,Peter Donnelly,Catherine Green,Simon R MyersPubMed 32744506
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg/mL | Notes The microwave method is recommended. Antigen retrieval is not essential but may optimise staining. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A
Recommended products
Mouse Polyclonal Rad51 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 55 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51. Database link Q06609
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
- Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
- Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
- Associated diseases and disorders
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
All lanes: Human colon lysate at 50 µg
Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody - BSA and Azide free (ab88572)
Immunofluorescent staining of Rad51 in HeLa cells using ab88572 at 10 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody - BSA and Azide free (ab88572)
ab88572 at 3μg/ml staining Rad51 in formalin-fixed, paraffin-embedded Human testis tissue.
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
All lanes: Rat PC-12 cell lysate at 50 µg
Predicted band size: 36 kDa
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
All lanes: Raw 264.7 cell lysate (Mouse) at 50 µg
Predicted band size: 36 kDa, 46 kDa
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
All lanes: Human Jurkat cell lysate at 50 µg
Predicted band size: 36 kDa
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
All lanes: NIH 3T3 cell lysate (Mouse) at 50 µg
Predicted band size: 36 kDa
Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572)
All lanes: Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL
Lane 1: transfected 293T cell lysate at 25 µg
Lane 2: non transfected lysate at 25 µg
Predicted band size: 36 kDa
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