AB88572

Anti-Rad51 antibody - BSA and Azide free

Name: Anti-Rad51 antibody - BSA and Azide free (ab88572) | Abcam
Brand: Abcam Limited
SKU: AB88572
Price: 450 USD
Availability: InStock
Rating: 4 (13 reviews)

(13 Reviews)

(67 Publications)

Mouse Polyclonal Rad51 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 67 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51.

View Alternative Names

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody - BSA and Azide free (AB88572)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody - BSA and Azide free (AB88572)

ab88572 at 3μg/ml staining Rad51 in formalin-fixed, paraffin-embedded Human testis tissue.

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody - BSA and Azide free (AB88572)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Immunofluorescent staining of Rad51 in HeLa cells using ab88572 at 10 μg/ml.

Unknown

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

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Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

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NIH 3T3 cell lysate (Mouse) at 50 µg

Predicted band size: 36 kDa

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Unknown

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

All lanes:

Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

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Raw 264.7 cell lysate (Mouse) at 50 µg

Predicted band size: 36 kDa,46 kDa

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Unknown

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

All lanes:

Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

Lane 1:

transfected 293T cell lysate at 25 µg

Lane 2:

non transfected lysate at 25 µg

Predicted band size: 36 kDa

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Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

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Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

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Human Jurkat cell lysate at 50 µg

Predicted band size: 36 kDa

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Unknown

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

All lanes:

Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

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Rat PC-12 cell lysate at 50 µg

Predicted band size: 36 kDa

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Unknown

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

All lanes:

Western blot - Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/mL

All lanes:

Human colon lysate at 50 µg

Predicted band size: 36 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAD51.

Q06609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "The microwave method is recommended.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.

Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 22325354, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 32640219). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed : 12205100, PubMed : 18417535, PubMed : 15226506, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 38459011). Recruited to resolve stalled replication forks during replication stress (PubMed : 27797818, PubMed : 31844045). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR (PubMed : 12442171, PubMed : 24141787). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (PubMed : 20413593). Also involved in interstrand cross-link repair (PubMed : 26253028).

See full target information RAD51

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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Current research in toxicology 8:100231 PubMed40237000

2025

Effects of mineral fibres in an in vitro placental syncytiotrophoblast model.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Tossetta,Sonia Fantone,Antonio Domenico Procopio,Armanda Pugnaloni,Alessandro Francesco Gualtieri,Daniela Marzioni

Journal of gastrointestinal oncology 16:191-208 PubMed40115921

2025

PRMT5 attenuates regorafenib-induced DNA damage in hepatocellular carcinoma cells through symmetric dimethylation of RPL14.

Applications

Unspecified application

Species

Unspecified reactive species

Wendi Bi,Xiaojuan Sun,Qiuyun Yi,Xinyu Jiang,Huisi He,Lixuan Jiang,Zhecai Fan,Hailing Huang,Wen Wen,Xiaoqing Jiang

Nature communications 16:1431 PubMed39920158

2025

D-2-hydroxyglutarate impairs DNA repair through epigenetic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Fengchao Lang,Karambir Kaur,Haiqing Fu,Javeria Zaheer,Diego Luis Ribeiro,Mirit I Aladjem,Chunzhang Yang

Animals : an open access journal from MDPI 14: PubMed39595299

2024

The Germline-Restricted Chromosome of Male Zebra Finches in Meiotic Prophase I: A Proteinaceous Scaffold and Chromatin Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey Matveevsky

Cell genomics 4:100550 PubMed38697125

2024

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Wang,Xinyi Liu,Xianbo Zuo,Cuiling Wang,Zheng Zhang,Haitao Zhang,Tao Zeng,Shunqi Chen,Mengyu Liu,Hongxia Chen,Qingfeng Song,Qi Li,Chenning Yang,Yi Le,Jinliang Xing,Hongxin Zhang,Jiaze An,Weihua Jia,Longli Kang,Hongxing Zhang,Hui Xie,Jiazhou Ye,Tianzhun Wu,Fuchu He,Xuejun Zhang,Yuanfeng Li,Gangqiao Zhou

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310146 PubMed38526153

2024

Targeting the KAT8/YEATS4 Axis Represses Tumor Growth and Increases Cisplatin Sensitivity in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miner Xie,Liwen Zhou,Ting Li,Yujie Lin,Ruhua Zhang,Xianchong Zheng,Cuiling Zeng,Lisi Zheng,Li Zhong,Xiaodan Huang,Yezi Zou,Tiebang Kang,Yuanzhong Wu

Cell death discovery 9:385 PubMed37863897

2023

YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Ai Chang,Rui-Zhi Ou Yang,Jing-Ming Su,Thi My Hang Nguyen,Junne-Ming Sung,Ming-Jer Tang,Wen-Tai Chiu

Cell death discovery 9:291 PubMed37558683

2023

Polymerase iota (POLI) confers radioresistance of esophageal squamous cell carcinoma by regulating RAD51 stability and facilitating homologous recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Li,Dexuan Gao,Fei Shen,Hengrui Chen,Zhuqiang Zhang,Chao He,Aidi Gao,Yue Lang,Xiaozhong Zhu,Jundong Zhou,Zeng-Fu Shang,Wei-Qun Ding,Ji Zhu

Cell reports 42:112329 PubMed37014751

2023

ERCC6L2 mitigates replication stress and promotes centromere stability.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Carnie,Lucy Armstrong,Marek Sebesta,Antonio Ariza,Xiaomeng Wang,Emily Graham,Kang Zhu,Dragana Ahel

RNA biology 19:1153-1171 PubMed36382943

2022

Early recruitment of PARP-dependent mA RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Soňa Legartová,Alena Svobodová Kovaříková,Jana Běhalová Suchánková,Hana Polášek-Sedláčková,Eva Bártová

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rad51 antibody - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Western blot - Anti-Rad51 antibody - BSA and Azide free (AB88572)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (67)

Product promise