Name: Anti-Rad51 antibody [EPR4030(3)]
SKU: ab133534
Rating: 4 (15 reviews)

Anti-Rad51 antibody [EPR4030(3)] is a rabbit recombinant monoclonal antibody that is used to detect Rad51 in Flow cytometry (Intra), ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 150 publications

Related conjugates and formulations

ab221796
Carrier free
Anti-Rad51 antibody [EPR4030(3)] - BSA and Azide free
ab303002
Conjugated
PE Anti-Rad51 antibody [EPR4030(3)]
ab303003
Conjugated
APC Anti-Rad51 antibody [EPR4030(3)]
ab303004
Conjugated
HRP Anti-Rad51 antibody [EPR4030(3)]
ab308698
Conjugated
Alkaline Phosphatase Anti-Rad51 antibody [EPR4030(3)]
ab310418
Conjugated
Alexa Fluor® 594 Anti-Rad51 antibody [EPR4030(3)]
ab309674
Conjugated
Alexa Fluor® 488 Anti-Rad51 antibody [EPR4030(3)]
ab310037
Conjugated
Alexa Fluor® 647 Anti-Rad51 antibody [EPR4030(3)]
ab311945
Conjugated
Alexa Fluor® 555 Anti-Rad51 antibody [EPR4030(3)]
ab312416
Conjugated
Alexa Fluor® 568 Anti-Rad51 antibody [EPR4030(3)]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Expected	Expected
Rat	Expected	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Rat	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes For unpurified use at 1/100 - 1/250.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/1000	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

See full target information RAD51

Function

The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4030(3)
Purification technique: Affinity purification Protein A
Dissociation constant: 9.2 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Anti-Rad51 antibody [EPR4030(3)] (ab133534) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.

Anti-Rad51 antibody [EPR4030(3)] (ab133534) was first used in a scientific publication in 2013 and has been cited over 151 times in peer reviewed journals. It's performance in Western Blot and immunofluorescence in human samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Rad51 antibody [EPR4030(3)] (ab133534) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Rad51 antibody [EPR4030(3)] (ab133534) has 13 independent reviews from customers.

Anti-Rad51 antibody [EPR4030(3)] (ab133534) specifically detects Rad51 (UniProt ID: Q06609; Molecular weight: 37kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR4030(3) - Anti-Rad51 antibody [EPR4030(3)] - BSA and Azide free ab221796.

Antibody clone EPR4030(3) is also available pre-conjugated to a variety of labels for your convenience - PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor^® 488, Alexa Fluor^® 647, Alexa Fluor^® 594, Alexa Fluor^® 555, Alexa Fluor^® 568, Alexa Fluor^® 750 (PE Anti-Rad51 antibody [EPR4030(3)] ab303002, APC Anti-Rad51 antibody [EPR4030(3)] ab303003, HRP Anti-Rad51 antibody [EPR4030(3)] ab303004, Alkaline Phosphatase Anti-Rad51 antibody [EPR4030(3)] ab308698, Alexa Fluor® 488 Anti-Rad51 antibody [EPR4030(3)] ab309674, Alexa Fluor® 647 Anti-Rad51 antibody [EPR4030(3)] ab310037, Alexa Fluor® 594 Anti-Rad51 antibody [EPR4030(3)] ab310418, Alexa Fluor® 555 Anti-Rad51 antibody [EPR4030(3)] ab311945, Alexa Fluor® 568 Anti-Rad51 antibody [EPR4030(3)] ab312416, Alexa Fluor® 750 Anti-Rad51 antibody [EPR4030(3)] ab321050).

Highly-cited antibody with >250 citations. A highly validated Rad51 antibody, essential for researchers studying DNA repair and homologous recombination. This antibody is crucial in genome stability research, particularly in understanding the DNA damage response. It is widely used in studies of Rad51 foci, cancer therapy and Rad51 inhibitors.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.

Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

Associated diseases and disorders

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling Rad51 with purified ab133534 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
ab133534 (purified) at 1/100 immunoprecipitating Rad51 in HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate.
Lane 1 (input): HEK-293 whole cell lysate (10μg)
Lane 2 (+): ab133534 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab133534 in HEK-293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Predicted band size: 36 kDa
Observed band size: 37 kDa
Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution
Lane 1: C6 (rat glial tumor cell line) whole cell lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
Flow Cytometry (Intracellular) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Intracellular Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Rad51 with purified ab133534 at 1/350 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Gao et al Nat Commun. 2018; 9: 3925. Published online 2018 Sep 25. doi: 10.1038/s41467-018-06407-7
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Immunocytochemical analysis of DT40 cells (WT and the indicated KOs) labeling Rad51 using ab133534 at 1/200 dilution.
From Figure 5d of gao et al.
Gao et al. Nat Commun. 2018; 9: 3925. Published online 2018 Sep 25. Published online 2018 Sep 25. doi: 10.1038/s41467-018-06407-7; PMID: 30254264
Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/.
Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution
All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Different batches of ab133534 were tested on HEK-293 (Human embryonic kidney epithelial cell) lysate at 2.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 37 kDa.
All lanes: Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Predicted band size: 36 kDa
Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/20000 dilution
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 21 kDa, 36 kDa
Observed band size: 22 kDa, 37 kDa
Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
All lanes: Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution
Lane 1: C6 (rat glial tumor cell line) cell lysate at 10 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 5: K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Rad51 with unpurified ab133534 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab133534 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab133534, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluorr^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 μg/1x10⁶ cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
OI-RD Scanning - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Rad51 Immunocytochemistry/ Immunofluorescence staining of NIH/3T3 cell line using rabbit Anti-Rad51 antibody
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling RAD51 with ab133534 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green).
The counterstain was observed in magenta.
Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Immunohistochemical analysis of formalin fixed paraffin embedded human testis labelling Rad51 with ab133534 at a concentration of 1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab133534 Anti-Rad51 antibody [EPR4030(3)] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
Rad51 Immunocytochemistry/ Immunofluorescence staining of HeLa cell line using rabbit Anti-Rad51 antibody
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling RAD51 with ab133534 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in HeLa cell line (shown in green).
The counterstain was observed in magenta.
Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.