Mouse Monoclonal Rad51 antibody. Suitable for Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAD51 aa 1-150.
Images
![Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (AB269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--flow-cytometry-img80349.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (AB269677)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (AB269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25736.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (AB269677)")
![Protein Array - Anti-Rad51 antibody [RAD51/2702] (AB269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--protein-array-img141313.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Rad51 antibody [RAD51/2702] (AB269677)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human RAD51 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q06609
Reactivity data
Select an application| Flow Cyt | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
5 products for Alternative Product
Target data
Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
Alternative names
DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A, RAD51A, RAD51, RECA
Recommended products
Mouse Monoclonal Rad51 antibody. Suitable for Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RAD51 aa 1-150.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human RAD51 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q06609
- Clone number
RAD51/2702
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
- Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
- Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
- Associated diseases and disorders
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--flow-cytometry-img80349.jpg "Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269677)")
Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269677)
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Rad51 using ab269677 ar 2 μg/ml (Blue) compared to an isotype control (Red). The secondary antibody is a Goat anti-Mouse IgG-CF488 conjugate.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25736.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269677)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Rad51 using ab269677 at 2 μg/ml in immunohistochemical analysis.
![Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269677), expandable thumbnail](https://content.abcam.com/products/images/rad51-antibody-rad51-2702-ab269677--protein-array-img141313.jpg "Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269677)")
Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269677)
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269677.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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