AB111568

Anti-Rad51 (phospho T309) antibody

Name: Anti-Rad51 (phospho T309) antibody (ab111568) | Abcam
Brand: Abcam Limited
SKU: AB111568
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Rad51 phospho T309 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RAD51 phospho T309.

View Alternative Names

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 (phospho T309) antibody (AB111568)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 (phospho T309) antibody (AB111568)

ab111568, at 1/50 dilution, staining Rad51 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human RAD51 phospho T309. The exact immunogen used to generate this antibody is proprietary information.

Q06609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111568 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.

Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 22325354, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 32640219). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed : 12205100, PubMed : 18417535, PubMed : 15226506, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 38459011). Recruited to resolve stalled replication forks during replication stress (PubMed : 27797818, PubMed : 31844045). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR (PubMed : 12442171, PubMed : 24141787). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (PubMed : 20413593). Also involved in interstrand cross-link repair (PubMed : 26253028).

See full target information RAD51 phospho T309

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:7554-7570 PubMed34197606

2021

PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Peng,Ruifeng Shi,Jing Bian,Yuwei Li,Peipei Wang,Hailong Wang,Ji Liao,Wei-Guo Zhu,Xingzhi Xu

Clinical epigenetics 11:4 PubMed30616689

2019

The histone methyltransferase DOT1L is required for proper DNA damage response, DNA repair, and modulates chemotherapy responsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Vijayalakshmi Kari,Sanjay Kumar Raul,Jana Maria Henck,Julia Kitz,Frank Kramer,Robyn Laura Kosinsky,Nadine Übelmesser,Wael Yassin Mansour,Jessica Eggert,Melanie Spitzner,Zeynab Najafova,Holger Bastians,Marian Grade,Jochen Gaedcke,Florian Wegwitz,Steven A Johnsen

Experimental and therapeutic medicine 14:2171-2179 PubMed28962138

2017

Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Bing Wang,Lei Li,Yawei Li,Pengzhi Li,Guohua Lv

Cell death & disease 7:e2383 PubMed27685627

2016

CHK1 and RAD51 activation after DNA damage is regulated via urokinase receptor/TLR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Pavan B Narayanaswamy,Sergey Tkachuk,Hermann Haller,Inna Dumler,Yulia Kiyan

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Rad51 (phospho T309) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 (phospho T309) antibody (AB111568)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise