Rabbit Polyclonal Rad51 phospho Y315 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RAD51 phospho Y315 aa 250 to C-terminus.
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- The Tohoku journal of experimental medicine 257:225-2392022Long Noncoding RNA Solute Carrier Family 25 Member 21 Antisense RNA 1 Inhibits Cell Malignant Behaviors and Enhances Radiosensitivity of Gastric Cancer Cells by Upregulating Synuclein Gamma Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wang,Zhiqiang Wang,Lin WangPubMed 35444105
- Cell death & disease 7:e23832016CHK1 and RAD51 activation after DNA damage is regulated via urokinase receptor/TLR4 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavan B Narayanaswamy et. al.PubMed 27685627
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human RAD51 phospho Y315 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q06609
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
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Target data
Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
Alternative names
DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A, RECA, RAD51A, RAD51
Recommended products
Rabbit Polyclonal Rad51 phospho Y315 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RAD51 phospho Y315 aa 250 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human RAD51 phospho Y315 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q06609
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
- Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
- Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
- Associated diseases and disorders
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Rad51 (phospho Y315) antibody (ab61111)
All lanes: Western blot - Anti-Rad51 (phospho Y315) antibody (ab61111) at 1/500 dilution
All lanes: Extract from Jurkat cells
Predicted band size: 36 kDa
Observed band size: 37 kDa
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