AB88370

Anti-RAD51AP1 antibody

Name: Anti-RAD51AP1 antibody (ab88370) | Abcam
Brand: Abcam Limited
SKU: AB88370
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Mouse Polyclonal RAD51AP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51AP1.

View Alternative Names

PIR51, RAD51AP1, RAD51-associated protein 1, HsRAD51AP1, RAD51-interacting protein

3 Images

Unknown

Western blot - Anti-RAD51AP1 antibody (AB88370)

All lanes:

Western blot - Anti-RAD51AP1 antibody (ab88370) at 1 µg/mL

Lane 1:

RAD51AP1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Predicted band size: 38 kDa

Observed band size: 37 kDa

false

Unknown

Western blot - Anti-RAD51AP1 antibody (AB88370)

All lanes:

Western blot - Anti-RAD51AP1 antibody (ab88370) at 1 µg/mL

All lanes:

Human colon tissue lysate at 50 µg

Predicted band size: 38 kDa

Observed band size: 40 kDa

false

CiteAb

Western blot - Anti-RAD51AP1 antibody (AB88370)

RAD51AP1 western blot using anti-RAD51AP1 antibody ab88370. Publication image and figure legend from Liang, F., Miller, A. S., et al., 2019, Nat Commun, PubMed 31253762.

ab88370 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab88370 please see the product overview.

Role of UAF1 and RAD51AP1 in FANCD2 deubiquitination in cells. a HeLa cells depleted for endogenous UAF1 and stably expressing UAF1 (WT), UAF13A (3A), or UAF111A (11A) were examined for their level of ubiquitinated FANCD2 by western blot. Ku86 was included as the loading control. b Survival of cells (from a) upon treatment with MMC or olaparib. The error bars represent mean values±S.D. of data from three independent experiments. c RAD51AP1 knockout HeLa cells depleted for endogenous UAF1 and stably expressing WT or mutant UAF1 with or without the co-expression of either RAD51AP1 (AP1) or the RAD51AP1DM (DM) mutant were examined for their level of ubiquitinated FANCD2. Ku86 was included as the loading control. The error bars represent the mean+S.D. of data from three independent experiments. d Survival of cells (from c) upon treatment with MMC. The error bars represent mean values±S.D. of data from three independent experiments. e Model for the role of the USP1-UAF1-RAD51AP1 complex in the FA pathway. Mono-ubiquitination and deubiquitination of FANCD2 both occur on DNA. DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the trimeric DUB complex to DNA bound, mono-ubiquitinated FANCD2

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RAD51AP1.

Q96B01

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 associated protein 1 also called RAD51AP1 is a protein with a molecular mass of approximately 41 kDa. It interacts directly with RAD51 to assist in DNA repair processes. RAD51AP1 is present in a variety of tissues with higher expression levels seen in testes and ovaries. This protein localizes in the nucleus where it takes part in processes involving DNA repair and stability.

Biological function summary

RAD51AP1 plays an important role in homologous recombination a critical process for the repair of double-strand breaks in DNA. It forms a complex with RAD51 to facilitate the homologous pairing and strand exchange steps of recombination. This interaction helps stabilize the RAD51 nucleoprotein filament which is important for successful recombination events. Apart from RAD51 it also interacts with other proteins involved in DNA repair pathways highlighting its collaborative function in maintaining genomic integrity.

Pathways

RAD51AP1 operates within the DNA damage repair pathway specifically in the homologous recombination repair mechanism. This pathway ensures that DNA double-strand breaks are accurately repaired preventing genomic aberrations. In this pathway the protein works in coordination with RAD51 and other recombinase proteins to facilitate error-free repair. RAD51AP1 also displays a connection to BRCA1 pathways; BRCA1 is another critical player in DNA repair and maintaining genomic stability.

RAD51AP1 has implications in cancer particularly breast and ovarian cancers. Mutations or dysregulation of RAD51AP1 may compromise the DNA repair mechanism leading to increased genomic instability and cancer susceptibility. Furthermore it relates to BRCA1 since both are involved in DNA repair pathways and mutations in BRCA1 are known to significantly elevate cancer risks. Understanding RAD51AP1 interactions and functions offers potential insights into targeted cancer therapies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination (PubMed : 17996710, PubMed : 17996711, PubMed : 20871616, PubMed : 25288561, PubMed : 26323318). Acts by stimulating D-Loop formation by RAD51 : specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51 (PubMed : 17996710, PubMed : 17996711). Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures : has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation (PubMed : 17996710, PubMed : 17996711, PubMed : 22375013, PubMed : 9396801). Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination : both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair (PubMed : 27239033, PubMed : 27463890, PubMed : 32350107). WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2 (PubMed : 31253762). Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination (PubMed : 21307306). Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination (PubMed : 31400850). May also bind RNA; additional evidences are however required to confirm RNA-binding in vivo (PubMed : 9396801).

See full target information RAD51AP1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 70:722-732 PubMed37962862

2023

The gene RAD51AP1 promotes the progression of pancreatic cancer via the PI3K/Akt/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongkun Wang,Yingchun Li,Cui Ran,Wenjun Le,Jiaxing Dong,Xujing Wang,Bo Chen,Xiaohua Jiang

Discover. Oncology 13:101 PubMed36197550

2022

Knockdown of RAD51AP1 suppressed cell proliferation and invasion in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang-Yang Hu,Chen-Chao Ma,Kai-Xing Ai

Nature communications 10:2849 PubMed31253762

2019

DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Fengshan Liang,Adam S Miller,Simonne Longerich,Caroline Tang,David Maranon,Elizabeth A Williamson,Robert Hromas,Claudia Wiese,Gary M Kupfer,Patrick Sung

Nature communications 10:65 PubMed30622262

2019

Promotion of homology-directed DNA repair by polyamines.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ying Lee,Guan-Chin Su,Wen-Yen Huang,Min-Yu Ko,Hsin-Yi Yeh,Geen-Dong Chang,Sung-Jan Lin,Peter Chi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAD51AP1 antibody

Western blot - Anti-RAD51AP1 antibody (AB88370)

Western blot - Anti-RAD51AP1 antibody (AB88370)

Western blot - Anti-RAD51AP1 antibody (AB88370)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise