Mouse Polyclonal Rad51C antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51C.
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Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51C. Database link O43502
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD51 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment; CX3 seems to act downstream of RAD51 recruitment; both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.
Alternative names
RAD51L2, RAD51C, DNA repair protein RAD51 homolog 3, R51H3, RAD51 homolog C, RAD51-like protein 2
Recommended products
Mouse Polyclonal Rad51C antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD51C.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RAD51C. Database link O43502
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad51C also known as RAD51 homolog C is a protein involved in the repair of DNA double-strand breaks through homologous recombination. It plays a mechanical role by forming nucleoprotein filaments essential for DNA pairing and strand exchange processes. The mass of Rad51C is approximately 42 kDa. This protein expresses widely in various tissues including ovaries and testes indicating its importance in genomic stability and cell division.
- Biological function summary
Rad51C contributes to maintaining genomic integrity by participating in the formation of the Rad51 paralog complex. This complex is essential in the repair of DNA damage and supports replication fork stability. Rad51C collaborates with other paralogs such as Rad51B Rad51D and XRCC2 forming the BCDX2 complex which promotes homologous recombination and protects against genotoxic stress. This involvement highlights its critical role in cellular defense mechanisms against DNA damage.
- Pathways
The protein participates actively in the homologous recombination repair pathway and the DNA damage response pathway. Within these pathways Rad51C interacts with pivotal genes such as BRCA1 BRCA2 and PALB2 facilitating repair of DNA damages and coordinating cellular response to replication stress. The interactions with these proteins form a network that orchestrates precise DNA repair processes ensuring cellular survival and minimization of mutations.
- Associated diseases and disorders
Rad51C connections include hereditary breast and ovarian cancer syndrome and Fanconi anemia-like phenotypes. Mutations or deficiencies in Rad51C can lead to compromised DNA repair raising the risk of these malignancies. In the context of cancer Rad51C operates alongside BRCA1 and BRCA2 where dysfunctions may result in elevated susceptibility to these conditions. Recognizing these interactions aids in understanding cancer pathogenesis and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Rad51C antibody (ab172270)
All lanes: Western blot - Anti-Rad51C antibody (ab172270) at 1 µg/mL
Lane 1: Rad51C-transfected 293T cell line lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
Developed using the ECL technique.
Predicted band size: 42 kDa
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