Anti-Rad51C antibody [EPR30494-537]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rad51C antibody [EPR30494-537] (AB325261)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 (human embryonic kidney epithelial cell); IMR-90 (human lung fibroblast) cells labelling Rad51C with ab325261 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in HEK-293 cells and weak nuclear staining in IMR-90 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Low expression : IMR-90 (PMID : 24742710)

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab325261 at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-Rad51C antibody [EPR30494-537] (AB325261)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : IMR-90 (PMID : 24742710).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Rad51C antibody [EPR30494-537] (ab325261) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 3:

IMR-90 (human lung fibroblast) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Rad51C antibody [EPR30494-537] (AB325261)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 24742710).

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the bands above 50 kDa and below 25kda (in lane 1) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Rad51C antibody [EPR30494-537] (ab325261) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 50 µg

Lane 2:

Human liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 38 kDa,36 kDa

true

Exposure time: 180s