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AB117097

Anti-RAD52 antibody

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(2 Publications)

Rabbit Polyclonal RAD52 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RAD52 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DNA repair protein RAD52 homolog, RAD52

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD52 antibody (AB117097)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD52 antibody (AB117097)

ab117097, at 2.5ug/ml, staining Human Prostate by Immunohistochemistry (Formalin-fixed, Paraffin-embedded)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human RAD52 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P43351

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 1.17% Sodium chloride, 0.424% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD52 also known by alternate names such as DNA repair protein RAD52 homolog functions as an important player in DNA repair mechanisms. It aids in the homologous recombination and repair of DNA double-strand breaks. RAD52 has a molecular mass of approximately 49 kDa and is mainly expressed in the nucleus. Its ability to bind to DNA and facilitate strand annealing are important for its mechanical function. RAD52 expression varies across tissues but it is prominently observed in rapidly dividing cells and tissues with high rates of DNA repair activity.
Biological function summary

DNA repair protein RAD52 homolog engages significantly in maintaining genome stability. RAD52 forms complexes with other DNA repair proteins such as RAD51 and RPA to perform its role efficiently. The protein supports the loading of RAD51 onto single-stranded DNA a critical step in homologous recombination. By stabilizing RAD51 nucleoprotein filaments RAD52 promotes efficient repair and recombination processes necessary for cellular survival after DNA damage.

Pathways

The activities of RAD52 link to the homologous recombination and DNA damage response pathways. RAD52 operates alongside proteins like RAD51 and BRCA2 to mediate the repair of DNA double-strand breaks through homologous recombination. This relationship is important for maintaining genomic integrity especially during the S and G2 phases of the cell cycle. The synergy between RAD52 and other double-strand break repair proteins ensures effective resolution of DNA damage.

The malfunction or dysregulation of RAD52 connects to conditions like cancer and Fanconi anemia. Deficiencies or mutations in RAD52 can compromise DNA repair and lead to genomic instability a hallmark of cancer. Additionally in Fanconi anemia the interplay between RAD52 and the FA complex is impaired influencing the DNA repair capacity. The disrupted interaction with other repair proteins including those in the BRCA family further suggests RAD52's important role in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
See full target information RAD52
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112043 PubMed36709426

2023

Elevated PAF1-RAD52 axis confers chemoresistance to human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Sanchita Rauth,Koelina Ganguly,Pranita Atri,Seema Parte,Rama Krishna Nimmakayala,Venkatesh Varadharaj,Palanisamy Nallasamy,Raghupathy Vengoji,Ayoola O Ogunleye,Imayavaramban Lakshmanan,Ramakanth Chirravuri,Mika Bessho,Jesse L Cox,Jason M Foster,Geoffrey A Talmon,Tadayoshi Bessho,Apar Kishor Ganti,Surinder K Batra,Moorthy P Ponnusamy

eLife 10: PubMed34318745

2021

An adhesion G protein-coupled receptor is required in cartilaginous and dense connective tissues to maintain spine alignment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Liu,Amro A Hussien,Yunjia Wang,Terry Heckmann,Roberto Gonzalez,Courtney M Karner,Jess G Snedeker,Ryan S Gray
View all publications
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Product promise

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